These Ptgs2 knock-out mice exhibit reduced viability with cardiac fibrosis and renal alterations characteristic of renal dysplasia. They are suitable for use in applications related to the study of mediators of inflammation.
Dr. Joe Dinchuk, The Dupont Merck Pharmaceutical Company
Mice homozygous for the Ptgs2tm1Jed targeted mutation exhibit significant preweaning loss of homozygotes (original publication reports 30-40%). Homozygous mutant mice show polydipsia and polyuria due to a defect in renal development. Cardiac fibrosis is evident in approximately 50% of the mice. PTGS2 deficient mice do not show altered inflammatory responses to in several tests of paw and ear edema; however, cytoxicity of hepatic cells induced by endotoxin was strikingly mitigated in these homozygotes. Female homozygotes are infertile with defects in ovulation, fertilization, implantation, and decidualization.
This strain was developed in the lab of Dr. Joe Dinchuk at Glenolden Laboratory, Dupont Merck Pharmaceutical Company. The transcription/translation start sites for the endogenous Ptgs2 gene have been removed. The 129S7-derived AB2.1 ES cell line was used. At The Jackson Laboratory, these mice were bred with B6129SF1/J (Stock No. 101043) to maintain the colony.
|Allele Name||targeted mutation 1, Joe E Dinchuk|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||COX-2-; COX2-; Ptgs2-|
|Gene Symbol and Name||Ptgs2, prostaglandin-endoperoxide synthase 2|
|Strain of Origin||129S7/SvEvBrd-Hprtb-m2|
|Molecular Note||A 1.8 kb genomic fragment containing exon 1 and the transcription and translation start sites was replaced with a neomycin selection cassette. Northern blot analysis on RNA derived from embryonic fibroblasts derived from homozygous embryos demonstrated that no detectable transcript was produced from this allele.|
|Mutations Made By|| |
Dr. Joe Dinchuk, The Dupont Merck Pharmaceutical Company
Homozygous males are fertile, females are infertile. They have a reported life expectancy of about 1 year. Homozygous males can be bred to heterozygous females. To increase litter sizes and the number of litters produced, a heterozygote x heterozygote breeding scheme may be used.
When using the COX-2- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002476 in your Materials and Methods section.
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