B6;129S4-Klf1^tm1Sho/J

Stock number: 002474
Product name: EKLF-
Research areas: Hematological Research

Description

Klf1 knock-out mice may be useful for studying human disease beta-thalassemia.

Detailed description

Homozygotes die at embryonic day 15 due to severe anemia during erythropoiesis of the fetal liver. There is a defect in beta-globin expression in these mice. This strain may serve as a model for the human disease beta-thalassemia.

Allele

Symbol: Klf1^tm1Sho
Name: targeted mutation 1, Stuart Orkin
MGI accession ID: MGI:1857162
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Klf1
Marker name: Kruppel-like factor 1 (erythroid)
Chromosome: 8
Strain of origin: 129S4/SvJae
Molecular note: A neomycin resistance cassette replaced a portion of intron 2 and exon 2, which encodes the zinc-finger DNA-binding domain of the protein. No stable RNA for the targeted gene was detected by RNase protection using RNA from homozygous mutant embryos.