Homozygotes die at embryonic day 15 due to severe anemia during erythropoiesis of the fetal liver. There is a defect in beta-globin expression in these mice. This strain may serve as a model for the human disease beta-thalassemia.
|Allele Name||targeted mutation 1, Stuart Orkin|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Klf1, Kruppel-like factor 1 (erythroid)|
|Strain of Origin||129S4/SvJae|
|Molecular Note||A neomycin resistance cassette replaced a portion of intron 2 and exon 2, which encodes the zinc-finger DNA-binding domain of the protein. No stable RNA for the targeted gene was detected by RNase protection using RNA from homozygous mutant embryos.|
|Mutations Made By|| |
Stuart Orkin, Harvard University
When maintaining a live colony, heterozygous mice may be bred to wildtype mice from the colony. Mice homozygous for the mutation are not viable.
When using the EKLF- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002474 in your Materials and Methods section.