These Prkcg knock-out mice exhibit diminished long-term potentiation in the hippocampus. They are suitable for use in applications related to the study of long-term potentiation in neuron signal transmission.
Dr. Susumu Tonegawa, Massachusetts Institute of Technology
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Prkcg | protein kinase C, gamma |
Mice homozygous for the Prkcgtm1Stl targeted mutation are viable and develop normally. Homozygous mice show diminished long-term potentiation (LTP) in the hippocampus, but two other types of synaptic plasticities are normal, namely long-term depression and paired-pulse facilitation. Despite this diminished LTP capacity homozygotes can learn to do hippocampus-dependant tasks, such as the Morris water maze and context-dependent fear conditioning, with only slight deficits.
The Prkcc deficient strain was developed in the lab of Dr. Susumu Tonegawa at the Center for Cancer Research at the Massachusetts Institute of Technology. The 129-derived E14 ES cell line was used.
Allele Name | targeted mutation 1, Susumu Tonegawa |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | PKCgamma-KO |
Gene Symbol and Name | Prkcg, protein kinase C, gamma |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 7 |
Molecular Note | A neomycin resistance cassette replaced 2.5kb of sequence containing the exon that encodes the nucleotide binding domain. Northern blot analysis of total brain RNA demonstrated that homozygous mutant mice lack normal transcripts. Western blot analysis of total brain protein did not detect the protein in homozygous mutant mice. |
Mutations Made By | Dr. Susumu Tonegawa, Massachusetts Institute of Technology |
Expected coat color from breeding:White Bellied Agouti, Black
When using the PKCγ KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #002466 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Prkcc<tm1Stl> |
Frozen Mouse Embryo | B6;129P2-Prkcg<tm1Stl>/J | $2595.00 |
Frozen Mouse Embryo | B6;129P2-Prkcg<tm1Stl>/J | $2595.00 |
Frozen Mouse Embryo | B6;129P2-Prkcg<tm1Stl>/J | $3373.50 |
Frozen Mouse Embryo | B6;129P2-Prkcg<tm1Stl>/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.