These double KO mice are deficient in low density lipoprotein receptor-related protein and low density lipoprotein receptor.
Dr. Joachim Herz, Univ of Texas Southwest Med Ctr Dallas
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Ldlr | low density lipoprotein receptor |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Lrpap1 | low density lipoprotein receptor-related protein associated protein 1 |
Mice homozygous for both the Ldlrtm1Her and Lrpap1tm1Her targeted mutations are viable and fertile. They have a functional deficiency of the low density lipoprotein receptor-related protein (LRP) and low density lipoprotein receptor (LDLR). LRP is reduced in both the liver and the brain of homozygotes. They also show impaired hepatic clearance of alpha2-macroglobulin and plasma accumulation of remnant lipoproteins.
This strain was developed in the lab of Dr. Joachim Herz at The University of Texas, Southwestern Medical Center at Dallas by mating Lrpap1-deficient and Ldlr-deficient mice.
Allele Name | targeted mutation 1, Joachim Herz |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | LDLR-; LDLR KO; LDLr0; LDLrKO; LDLr-KO; Ldlrtm1Her |
Gene Symbol and Name | Ldlr, low density lipoprotein receptor |
Gene Synonym(s) | |
Site of Expression | Immunoblot analysis of liver membranes detected a truncated protein in homozygous mutant animals. |
Strain of Origin | 129S7/SvEvBrd-Hprt+ |
Chromosome | 9 |
General Note | When used in bone marrow transplant into Ldlrtm1Her homozygous mice, Abca1tm1Jdm Abcg1tm1Dgen homozygous cells accelerate the development of atherosclerosis. (J:130777) Phenotypic Similarity to Human Syndrome: Atherosclerosis, Susceptibility to J:29950, J:225091 in mice fed a high-cholesterol diet. |
Molecular Note | Insertion of a neomycin resistance cassette into exon 4. The authors predict that the targeted allele would encode a truncated non-functional protein that will not bind LDL, and that lacks a membrane spanning segment. Immunoblot analysis of liver membranes detected a truncated protein in homozygous mutant animals. |
Mutations Made By | Dr. Joachim Herz, Univ of Texas Southwest Med Ctr Dallas |
Allele Name | targeted mutation 1, Joachim Herz |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | RAP- |
Gene Symbol and Name | Lrpap1, low density lipoprotein receptor-related protein associated protein 1 |
Gene Synonym(s) | |
Strain of Origin | 129S7/SvEvBrd |
Chromosome | 5 |
General Note | ES cell line = JH1 or AB1 (129S7/SvEvBrd-Hprt+). |
Molecular Note | A neomycin resistance cassette was inserted into sequences that encode the leader peptide. Immunoblot analysis of liver and brain did not detect the encoded protein in homozygous animals. |
Mutations Made By | Dr. Joachim Herz, Univ of Texas Southwest Med Ctr Dallas |
The strain is maintained by mating homozygous siblings.
When using the RAP- LDLR- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002465 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Homozygous for Ldlr<tm1Her>, Homozygous for Lrpap1<tm1Her> |
Frozen Mouse Embryo | B6;129S-Lrpap1<tm1Her> Ldlr<tm1Her>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6;129S-Lrpap1<tm1Her> Ldlr<tm1Her>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6;129S-Lrpap1<tm1Her> Ldlr<tm1Her>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6;129S-Lrpap1<tm1Her> Ldlr<tm1Her>/J Frozen Embryos | $3373.50 |
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