These MMTV-TGFα mice express a transgene containing the normal allele of the human transforming growth factor alpha gene and adult females exhibit sebaceous gland hyperplasia and inadequate lactation in females.
Dr. Robert J. Coffey, Vanderbilt University Medical Center
The phenotype of TgN(MMTVTGFA)254Rjc transgenic mice is very similar to that of B6D2-TgN(MMTVTGFA)29Rjc (Stock No. 002373). Mice are viable and fertile, but lactate insufficiently to support the litter. Virgin transgenic mice show no mammary gland abnormalities prepubertally, but adult virgin mice have considerable alveolar gland hyperplasia. Pregnant transgenic mice show marked proliferation of the stromal cells, and alveolar secretion is markedly increased compared to nontransgenic mice. After multiple pregnancies, isolated adenocarcinomas develop. There is no apparent phenotypic effect in males. In females transgene expression was localized to the small ducts and alveoli in both virgin and pregnant mice as evidenced by in situ hybridization and by immunohistochemistry. Immunostaining also revealed some stromal staining in the hyperplastic areas. Egfr mRNA expression was also increased in mammary tissues expressing high levels of the transgene. Crosses between TGFA transgenic mice and TGFB1 transgenic mice demonstrate that these growth factors oppose each other's action on mammary gland development. The B6D2-TgN(MMTVTGFA)254Rjc line is notable for sebaceous gland hyperplasia, which occurs in both male and female mice. The hyperplasia was evidenced as multiple raised, white plaques and nodules on the abdominal skin. No dysplasia or sebaceous cell carcinoma was observed. High expression of TGFa mRNA and TGFa protein was detected in the areas of sebaceous hyperplasia.
To construct pMMTV-TGFa, a pKCR vector in which the SV40 early promoter region was replaced with complete MMTV LTR and a 925 bp human TGFa cDNA was inserted in the EcoRI site of b-globin exon 3. The 3.6 kb XhoI fragment was microinjected into (C57BL X DBA)F2 fertilized eggs. Transgenic mice are identified either by Southern blot using a b-globin exon 3 probe or by PCR. Transgenic lines were generated by mating founder animals to (C57BL X DBA/2)F1 males and females. No details of subsequent strain development were provided.
|Expressed Gene||TGFA, transforming growth factor alpha, human|
|Site of Expression|
|Allele Name||transgene insertion 254, Robert J Coffey|
|Allele Type||Transgenic (Inserted expressed sequence, Humanized sequence)|
|Allele Synonym(s)||transgene insertion 254, Robert J Coffey; Tg(MMTVTGFA)254Rjc|
|Gene Symbol and Name||Tg(MMTVTGFA)254Rjc, transgene insertion 254, Robert J Coffey|
|Promoter||MMTV, Mouse Mammary Tumor Virus, MMTV|
|Expressed Gene||TGFA, transforming growth factor alpha, human|
|Strain of Origin||(C57BL/6 x DBA/2)F2|
|Molecular Note||The transgene contains the mouse mammary tumor virus LTR and the normal allele of the human transforming growth factor alpha gene. The vector also contains exons 2 and 3 of the rabbit beta-globin gene. Lines 254, 257, 64 and 29 were produced.|
|Mutations Made By|| |
Dr. Robert Coffey, Vanderbilt University Medical Center
When maintaining a live colony, this strain is maintained by mating a (C57BL/6J x DBA/2)F1 female with transgenic carrier male. Female transgenic mice do not lactate.
When using the MMTV-TGFα mouse strain in a publication, please cite the originating article(s) and include JAX stock #002459 in your Materials and Methods section.
|Carrier or non-carrier for Tg(MMTVTGFA)254Rjc|
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
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