These Tap1 knock-out mice exhibit a lack of lack CD4-8+ T cells and reduced levels of class I surface molecules.
Dr. Susumu Tonegawa, Massachusetts Institute of Technology
Mice homozygous mice for the Tap1tm1Arp targeted mutation are viable and fertile. They are defective in the stable assembly and intracellular transport of class I molecules. They show severely reduced levels of class I surface molecules. They lack CD4-8+ T cells. This phenotype is similar to the mouse RMA-S cell line and human beings with TAP1 and TAP2 deficiencies. This strain should be housed under pathogen free conditions similar to the Prkdcscid/scid mouse or any other immunodeficient strain.
The Tap1-deficient strain was developed by homologous recombination in D3 ES cells. It was developed in the laboratory of Dr. Susumu Tonegawa at MIT. A targeting vector was used that resulted in about 80% of the protein coding region. The 129/Sv-derived D3 ES cell line was used.
|Allele Name||targeted mutation 1, Philip Ashton-Rickardt|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Abcb2tm1Arp; TAP-; TAP-1-; TAP1-|
|Gene Symbol and Name||Tap1, transporter 1, ATP-binding cassette, sub-family B (MDR/TAP)|
|Strain of Origin||129S2/SvPas|
|Molecular Note||A 7kb region of the wild-type locus (which made up 80% of the protein coding region) was replaced by a neomycin resistance cassette.|
|Mutations Made By|| |
Dr. Philip Ashton-Rickardt, Imperial College London
When maintaining a live colony, these mice can be bred as homozygotes.
Tap1-deficient mice are immunodeficient. As such, and similar to other immunodeficient strains, maintenance in high health status (specific pathogen-free) vivaria promotes overall colony health. If these animals are maintained in low health barrier rooms, the use of medicated water (e.g., sulfatrim/trimethoprim-sulfa or enrofloxacin/Baytril) is suggested to increase overall colony health.
When using the TAP1- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002458 in your Materials and Methods section.
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