B6;129S2-Tap1^tm1Arp/J

Stock number: 002458
Product name: TAP1-
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

These Tap1 knock-out mice exhibit a lack of lack CD4^-8⁺ T cells and reduced levels of class I surface molecules.

Detailed description

Mice homozygous mice for the Tap1^tm1Arp targeted mutation are viable and fertile. They are defective in the stable assembly and intracellular transport of class I molecules. They show severely reduced levels of class I surface molecules. They lack CD4^-8⁺ T cells. This phenotype is similar to the mouse RMA-S cell line and human beings with TAP1 and TAP2 deficiencies. This strain should be housed under pathogen free conditions similar to the Prkdc^scid/scid mouse or any other immunodeficient strain.

Development

The Tap1-deficient strain was developed by homologous recombination in D3 ES cells. It was developed in the laboratory of Dr. Susumu Tonegawa at MIT. A targeting vector was used that resulted in about 80% of the protein coding region. The 129/Sv-derived D3 ES cell line was used.

Allele

Symbol: Tap1^tm1Arp
Name: targeted mutation 1, Philip Ashton-Rickardt
MGI accession ID: MGI:1857253
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Tap1
Marker name: transporter 1, ATP-binding cassette, sub-family B (MDR/TAP)
Chromosome: 17
Strain of origin: 129S2/SvPas
Molecular note: A 7kb region of the wild-type locus (which made up 80% of the protein coding region) was replaced by a neomycin resistance cassette.