In these Notch1 deficient mice, somitogenesis is disturbed and there is a probable defect in chorio-allantoic connection. Homozygotes die at embryonic day 9.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
The targeting vector used deleted a large portion of the Notch1 gene.
|Allele Name||targeted mutation 1, Ronald L Conlon|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||N1delta1; Notch-; Notchdelta1; Notch1-; Notch1tm1Con/J|
|Gene Symbol and Name||Notch1, notch 1|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||Deletion of sequences encoding the EGF repeats 28 to 36, three Notch repeats, the transmembrane domain and CDC10/SWI6 repeats, and replacement with a neomycin gene.|
|Mutations Made By|| |
Dr. Ronald Conlon, Case Western Reserve Univ, School of Med
This strain is maintained by breeding heterozygous mice to normal wildtype siblings. Expected coat color from breeding:Albino
When using the STOCK Notch1tm1Con/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #002445 in your Materials and Methods section.