These lckPr-bcl-2 mice express a transgene containing human apoptosis regulator (BCL2) cDNA and are resistant to apoptosis induced by glucocorticoid treatment, radiation treatment, or anti-CD3 treatment.
Dr. Stanley J. Korsmeyer, Dana-Farber Cancer Institute
This strain does not carry mouse mammary tumor virus (MMTV).
Hemizygotes carrying the human LCKprBCL2 transgene display normal architecture of all lymphoid organs to 10 weeks of age. They show an increased percentage of CD3hi/TCRhi and CD4-8+ thymocytes with a decreased percentage in CD3lo T cells. CD8+ cells and the total percentage of T cells are increased in the spleen and lymph nodes. Mice are resistant to apoptosis induced by glucocorticoid treatment, by radiation treatment, or by anti-CD3 treatment. Malignant lymphoma develop in hemizygotes at approximately 18 months of age. Hemizygous BCL2 transgenic mice are fertile.
|Expressed Gene||BCL2, BCL2, apoptosis regulator, human|
|Site of Expression|
|Allele Name||transgene insertion 36, Stanley J Korsmeyer|
|Allele Type||Transgenic (Inserted expressed sequence, Humanized sequence)|
|Allele Synonym(s)||lckpr-bcl-2; Lck-BCL2; Lck-Bcl-2; prLck.hbcl-2|
|Gene Symbol and Name||Tg(LCKprBCL2)36Sjk, transgene insertion 36, Stanley J Korsmeyer|
|Promoter||Lck, lymphocyte protein tyrosine kinase, mouse, laboratory|
|Expressed Gene||BCL2, BCL2, apoptosis regulator, human|
|Strain of Origin||Not Specified|
|Molecular Note||The transgene consists of 0.75 kb of Lck proximal promoter sequences (active in lymphoid tissues) and 3.2 kb of human BCL2 cDNA. Introns, exons, and polyadenylation sequences from the human growth hormone gene consitutes the 3' untranslated region of the construct. Transgene expression in the thymus, lymph nodes, and spleen was verified by Western blot analysis and immunohistochemistry. Seven transgenic lines were established with lines 7, 17, and 36 expressing the transgene at high levels.|
|Mutations Made By|| |
Dr. Stanley Korsmeyer, Dana-Farber Cancer Institute
When maintaining a live colony, hemizygous transgenic males may be bred to C3H/HeJ females (Stock No. 000659). The expected coat color from breeding is white bellied agouti.
When using the lckPr-bcl-2 mouse strain in a publication, please cite the originating article(s) and include JAX stock #002427 in your Materials and Methods section.
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