These Wap-ras mice express a transgene containing human Harvey rat sarcoma viral oncogene gene integrated into the SJL Y chromosome.
Dr. Loretta L. Nielsen, Amyllin Pharmaceuticals Inc.
The transgene integrated into the Y chromosome. These transgenics serve as models for male mammary cancer and ras-mediated solid tumors. The transgene also can be used as a Y-chromosome marker. On the B6,SJL background males develop multiple mammary tumors and salivary gland tumors by one year of age. The salivary tumors are adenocarcinomas arising from serous areas of the submandibular gland. The mammary gland tumors are adenosquamous carcinomas. Microscopic lung metastases were present in 14% of tumor bearing animals. On the FVB background males develop multiple mammary tumors at 6-8 weeks. The tumors are adenosquamous carcinomas with multiple foci of squamous differentiation or adenocarcinomas containing glandular tissue. There is no metastasis observed.
This transgenic strain was developed in the laboratory of Dr. Loretta Nielsen at Schering-Plough Research, Bloomfield, New Jersey. This strain was originally identified as Wap-ras line 69-2F.
|Expressed Gene||HRAS, HRas proto-oncogene, GTPase, human|
|Site of Expression|
|Allele Name||transgene insertion 69, Loretta L Nielsen|
|Allele Type||Transgenic (Inserted expressed sequence, Humanized sequence)|
|Allele Synonym(s)||HRAS; Tg(WapHRAS)69Lin; Tg(Wap-HRAS)69Lin|
|Gene Symbol and Name||Tg(Wap-HRAS)69Lln, transgene insertion 69, Loretta L Nielsen|
|Promoter||Wap, whey acidic protein, mouse, laboratory|
|Expressed Gene||HRAS, HRas proto-oncogene, GTPase, human|
|Strain of Origin||(C57BL/6 x SJL)F1|
|Molecular Note||This transgene contains the murine whey acidic protein promoter and an allele of the human Harvey rat sarcoma viral oncogene gene homolog that contains a point mutation in codon 12. The transgene integrated into the Y chromosome (SJL).|
|Mutations Made By|| |
Dr. Loretta Nielsen, Amyllin Pharmaceuticals Inc.
When maintaining a live colony, wildtype (non-carrier) females from the colony or FVB/NJ inbred females (Stock No. 001800) may be bred to hemizygous males.
When using the Wap-ras mouse strain in a publication, please cite the originating article(s) and include JAX stock #002410 in your Materials and Methods section.
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