Homozygous mutant mice have normal numbers of CD8+ T cells and NK cells. CTL and NK cells are unable to lyse virus-infected or allogeneic fibroblasts in vitro. Homozygotes fail to clear lymphocytic choriomeningitis virus. Fibrosarcoma tumor cells are eliminated with reduced efficiency.
Dr. Hans Hengartner, University Hospital Zurich
Genetic Background | Generation |
---|---|
F?+N3F3pF13
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Prf1 | perforin 1 (pore forming protein) |
Mice homozygous for this perforin-1 targeted mutation are viable and fertile. Homozygous mutant mice have normal numbers of CD8+ T cells and NK cells. CTL and NK cells are unable to lyse virus-infected or allogeneic fibroblasts in vitro. Homozygotes fail to clear lymphocytic choriomeningitis virus. Fibrosarcoma tumor cells are eliminated with reduced efficiency.
A replacement vector was used containing the neo gene and which resulted in the disruption of exon 3 of the perforin gene without deletion of coding sequence. No full perforin mRNA was detected by reverse transcription PCR. The targeting construct was introduced into BL/6-III ES cells, which are derived from C57BL/6 male mice.
Allele Name | targeted mutation 1, Sandoz Pharmaceuticals |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | P0; perf-; perforin-; perforin 0; Pfn-; pfp-; Pfptm1Sdz; pfpKO; pko; Prf-; Prf1-; Prf1tm/Sdz; prf1tm1 |
Gene Symbol and Name | Prf1, perforin 1 (pore forming protein) |
Gene Synonym(s) | |
Strain of Origin | C57BL/6J |
Chromosome | 10 |
Molecular Note | A neomycin selection cassette was inserted into exon 3. RT-PCR analysis on RNA derived from homozygous mice demonstrated that an abnormal transcript was produced from this allele. However, immunocytochemistry experiments on activated spleen cells derived from homozygous mice confirmed that no detectable protein was made from this allele. |
Mutations Made By | Dr. Birgit Lederman, University of Zurich |
This colony is maintained by breeding homozygous siblings; reproduction performance is similar to C57LB/6J. Young mutants are susceptible to mouse hepatitis virus, but after reaching about eight weeks of age, they survive well conventional animal housing conditions. The expected coat color from breeding is black.
When using the Prf1 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #002407 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous for Prf1<tm1Sdz> |
Frozen Mouse Embryo | C57BL/6-Prf1<tm1Sdz>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Prf1<tm1Sdz>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Prf1<tm1Sdz>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | C57BL/6-Prf1<tm1Sdz>/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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