C57BL/6-Prf1^tm1Sdz/J

Stock number: 002407
Product name: Prf1 KO
Research areas: Apoptosis Research, Immunology, Inflammation and Autoimmunity Research

Description

Homozygous mutant mice have normal numbers of CD8⁺ T cells and NK cells. CTL and NK cells are unable to lyse virus-infected or allogeneic fibroblasts in vitro. Homozygotes fail to clear lymphocytic choriomeningitis virus. Fibrosarcoma tumor cells are eliminated with reduced efficiency.

Detailed description

Mice homozygous for this perforin-1 targeted mutation are viable and fertile. Homozygous mutant mice have normal numbers of CD8⁺ T cells and NK cells. CTL and NK cells are unable to lyse virus-infected or allogeneic fibroblasts in vitro. Homozygotes fail to clear lymphocytic choriomeningitis virus. Fibrosarcoma tumor cells are eliminated with reduced efficiency. 

Development

A replacement vector was used containing the neo gene and which resulted in the disruption of exon 3 of the perforin gene without deletion of coding sequence. No full perforin mRNA was detected by reverse transcription PCR. The targeting construct was introduced into BL/6-III ES cells, which are derived from C57BL/6 male mice.

Allele

Symbol: Prf1^tm1Sdz
Name: targeted mutation 1, Sandoz Pharmaceuticals
MGI accession ID: MGI:1857235
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Prf1
Marker name: perforin 1 (pore forming protein)
Chromosome: 10
Strain of origin: C57BL/6
Molecular note: A neomycin selection cassette was inserted into exon 3. RT-PCR analysis on RNA derived from homozygous mice demonstrated that an abnormal transcript was produced from this allele. However, immunocytochemistry experiments on activated spleen cells derived from homozygous mice confirmed that no detectable protein was made from this allele.