Mice homozygous for the Csf3tm1Ard targeted mutation are viable and fertile but are characterized by chronic neutropenia. Peripheral blood neutrophil levels of homozygotes are 20 to 30% of wildtype. There is a 50% reduction of granulocyte, macrophage and blast progenitor cells in marrow of homozygous mice and an impaired resistance to infection with Listeria monocytogenes.
The Csf3-deficient strain was produced in the laboratory of Dr. Ashley Dunn from the Ludwig Institute for Cancer Research at The Royal Melbourne Hospital, Victoria, Australia. A replacement type targeting vector was used that resulted in the deletion of exons 1 and 2 and most of exon 3. E14 derived ES cells were used.
|Expressed Gene||lacZ, beta-galactosidase, E. coli|
|Site of Expression|
|Allele Name||targeted mutation 1, Ashley R Dunn|
|Allele Type||Targeted (Reporter, Null/Knockout)|
|Gene Symbol and Name||Csf3, colony stimulating factor 3 (granulocyte)|
|Expressed Gene||lacZ, beta-galactosidase, E. coli|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||A 0.7kb genomic fragment containing exons 1-3 was replaced by a lacZ gene and neomycin resistance cassette. This mutation placed the lacZ gene under the control of the Csf3 promoter elements. An activity assay demonstrated that the functional protein was not detectable in cultured medium derived from organ cultures of various tissues of homozygous mice.|
|Mutations Made By|| |
Dr. Ashley Dunn, Ludwig Institute for Cancer Research
The Csf3-deficient strain is maintained by homozygous sibling matings. This strain should be housed under pathogen free conditions similar to the Prkdcscid/Prkdcscid mouse or any other immunodeficient strain. It is on a mixed C57BL/6 x 129/Ola genetic background.
When using the G-CSF- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002398 in your Materials and Methods section.
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