B6;129P2-Csf3^tm1Ard/J

Stock number: 002398
Product name: G-CSF-
Research areas: Cancer Research, Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

These Csf3 knock-out mice exhibit chronic neutropenia.

Detailed description

Mice homozygous for the Csf3^tm1Ard targeted mutation are viable and fertile but are characterized by chronic neutropenia. Peripheral blood neutrophil levels of homozygotes are 20 to 30% of wildtype. There is a 50% reduction of granulocyte, macrophage and blast progenitor cells in marrow of homozygous mice and an impaired resistance to infection with Listeria monocytogenes.

Development

The Csf3-deficient strain was produced in the laboratory of Dr. Ashley Dunn from the Ludwig Institute for Cancer Research at The Royal Melbourne Hospital, Victoria, Australia. A replacement type targeting vector was used that resulted in the deletion of exons 1 and 2 and most of exon 3. 129P2/OlaHsd-derived E14 embryonic stem (ES) cells were used. This strain was maintained on a mixed C57BL/6 x 129/Ola genetic background prior to arrival at The Jackson Laboratory.

Allele

Symbol: Csf3^tm1Ard
Name: targeted mutation 1, Ashley R Dunn
MGI accession ID: MGI:1857155
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Synonyms: G-CSF-
Marker symbol: Csf3
Marker name: colony stimulating factor 3 (granulocyte)
Marker synonyms: C17orf33; CSF3OS; Csfg; Gcsf; G-CSF; MGI-IG
Chromosome: 11
Strain of origin: 129P2/OlaHsd
Expressed genes: lacZ,beta-galactosidase,E. coli
Molecular note: A 0.7kb genomic fragment containing exons 1-3 was replaced by a lacZ gene and neomycin resistance cassette. This mutation placed the lacZ gene under the control of the Csf3 promoter elements. An activity assay demonstrated that the functional protein was not detectable in cultured medium derived from organ cultures of various tissues of homozygous mice.