129-Cd5^tm1Cgn/J

Stock number: 002397
Product name: CD5T
Research areas: Immunology, Inflammation and Autoimmunity Research

Detailed description

Mice homozygous for the Cd5^tm1Cgn targeted mutation are viable and apparently healthy. Both T and B cells in homozygous mutant mice lack surface CD5. Mice show normal immune responses to both T cell-dependent and -independent antigens.

Allele

Symbol: Cd5^tm1Cgn
Name: targeted mutation 1, University of Cologne
MGI accession ID: MGI:1857148
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Cd5
Marker name: CD5 antigen
Chromosome: 19
Strain of origin: 129P2/OlaHsd
Molecular note: A neomycin gene replaced part of exon 7, which encodes the transmembrane domain. An additional frameshift mutation was introduced into exon 3. Flow cytometry analysis on cells derived from thymus, spleen and the peritoneal cavity of homozygous mice confirmed that no detectable protein was expressed from this allele.