B6;129S7-Src^tm1Sor/J

Stock number: 002381
Product name: src-
Research areas: Cancer Research, Developmental Biology Research, Internal/Organ Research

Description

These Src knock-out mice exhibit a deficiency in bone remodeling, indicating impaired osteoclast function, and developed osteopetrosis. They are suitable for use in applications related to the study of the physiological role of the Rous sarcoma oncogene.

Detailed description

Mice homozygous for the Src^tm1Sor targeted mutation display osteopetrosis. Homozygous mutant mice are approximately one-third the size of normal wildtype siblings. Incisors fail to erupt. In general, long bones are shorter in length and show a partial absence of bone marrow. Src has been implicated in development, but its role may be masked by other tyrosine kinases. No overt phenotype is found in brain or platelets, where it is most highly expressed. Mice heterozygous for the Src^tm1Sor mutation have no apparent abnormalities.

Allele

Symbol: Src^tm1Sor
Name: targeted mutation 1, Philippe Soriano
MGI accession ID: MGI:1857247
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Src
Marker name: Rous sarcoma oncogene
Chromosome: 2
Strain of origin: 129S7/SvEvBrd-Hprt^b-m2
Molecular note: A neomycin cassette was inserted into the first coding exon. Northern blot analysis confirmed that no wild-type transcript was present in homozygous mice. No autophosphorylation activity corresponding to the encoded protein is detectable in homozygous mice.