Homozygous NOD scid RipTag mice develop pancreatic beta cell adenomas that are free of autoimmune T-cells.
Dr. David Serreze, The Jackson Laboratory
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Spontaneous | Prkdc | protein kinase, DNA activated, catalytic polypeptide |
Allele Type |
---|
Transgenic (Inserted expressed sequence) |
Homozygous NOD scid RipTag mice (NOD/Lt-TgN(RipTag)1Lt Prkdcscid) develop pancreatic beta cell adenomas. Because Prkdcscid mice lack T-cells, the adenomas from this strain are free of the autoimmune T-cells found in the adenomas of NOD RipTag mice (Stock No. 002033).
Mice homozygous for the RipTag transgene are able to produce 2-3 litters before the insulin secreted from the adenoma makes them too hypoglycemic. If needed, transgenic mice can be supplemented with 5% sucrose in the drinking water to limit the development of hypoglycemia.
The TgN(RipTAg)1Lt transgene was generated in NOD/Lt. It was crossed with NOD.CB17-Prkdcscid by Dave Serreze and sibling bred to homozygosity for both the transgene and Prkdcscid.
Expressed Gene | TAg, SV40 large T-antigen, SV40 |
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Site of Expression |
Allele Name | severe combined immunodeficiency |
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Allele Type | Spontaneous |
Allele Synonym(s) | SCID |
Gene Symbol and Name | Prkdc, protein kinase, DNA activated, catalytic polypeptide |
Gene Synonym(s) | |
Site of Expression | T and B lymphocytes. |
Strain of Origin | C.BKa-Ighb/Icr |
Chromosome | 16 |
Molecular Note | A T-to-A transversion point mutation at a position corresponding to codon 4046 (codon 4095 in transcript ENSMUST00000023352.8) created a premature stop codon (p.Y4046*). |
Allele Name | transgene insertion 1, Edward H Leiter |
---|---|
Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | Tg(RipTAg)1Lt |
Gene Symbol and Name | Tg(Ins2-TAg)1Lt, transgene insertion 1, Edward H Leiter |
Gene Synonym(s) | |
Promoter | Ins2, insulin 2, rat |
Expressed Gene | TAg, SV40 large T-antigen, SV40 |
Strain of Origin | NOD/ShiLt |
Chromosome | UN |
General Note | Homozygous transgenic mice on an NOD/Lt genetic background develop pancreatic beta cell adenomas. They are able to produce 2-3 litters before the insulin secreted from the adenoma makes them too hypoglycemic. |
Molecular Note | The transgene contains rat insulin 2 promoter (Rip) and the wild-type allele of the SV40 large T antigen (TAg) gene. |
Mutations Made By | Dr. David Serreze, The Jackson Laboratory |
When maintaining a live colony, mice homozygous for the targeted mutation and hemizygous for the transgene (Tg/?) are bred together. If needed, transgenic mice can be supplemented with 5% sucrose in the drinking water to limit the development of hypoglycemia. Expected coat color from breeding is albino.
When using the NOD scid RipTag mouse strain in a publication, please cite the originating article(s) and include JAX stock #002380 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous for Tg(Ins2-TAg)1Lt, Homozygous for Prkdc<scid> |
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