NOD.Cg-Tg(Ins2-TAg)1Lt Prkdc^scid/DvsJ

Stock number: 002380
Product name: NOD scid RipTag
Research areas: Cancer Research, Diabetes and Obesity Research, Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research, Research Tools, Virology Research

Description

Homozygous NOD scid RipTag mice develop pancreatic beta cell adenomas that are free of autoimmune T-cells.

Detailed description

Homozygous NOD scid RipTag mice (NOD/Lt-TgN(RipTag)1Lt Prkdc^scid) develop pancreatic beta cell adenomas. Because Prkdc^scid mice lack T-cells, the adenomas from this strain are free of the autoimmune T-cells found in the adenomas of NOD RipTag mice (Stock No. 002033).
Mice homozygous for the RipTag transgene are able to produce 2-3 litters before the insulin secreted from the adenoma makes them too hypoglycemic. If needed, transgenic mice can be supplemented with 5% sucrose in the drinking water to limit the development of hypoglycemia.

Development

The TgN(RipTAg)1Lt transgene was generated in NOD/Lt. It was crossed with NOD.CB17-Prkdc^scid by Dave Serreze and sibling bred to homozygosity for both the transgene and Prkdc^scid.

Allele

Symbol: Tg(Ins2-TAg)1Lt
Name: transgene insertion 1, Edward H Leiter
MGI accession ID: MGI:3052662
Generation method: Transgenic
Attributes: Inserted expressed sequence
Synonyms: Tg(RipTAg)1Lt
Marker symbol: Tg(Ins2-TAg)1Lt
Marker name: transgene insertion 1, Edward H Leiter
Marker synonyms: Tg(RipTAg)1Lt
Chromosome: UN
Strain of origin: NOD/ShiLt
Expressed genes: TAg,SV40 large T-antigen,SV40
Molecular note: The transgene contains rat insulin 2 promoter (Rip) and the wild-type allele of the SV40 large T antigen (TAg) gene.
General note: Homozygous transgenic mice on an NOD/Lt genetic background develop pancreatic beta cell adenomas. They are able to produce 2-3 litters before the insulin secreted from the adenoma makes them too hypoglycemic.

Allele

Symbol: Prkdc^scid
Name: severe combined immunodeficiency
MGI accession ID: MGI:1857113
Generation method: Spontaneous
Synonyms: scid
Marker symbol: Prkdc
Marker name: protein kinase, DNA activated, catalytic polypeptide
Marker synonyms: DNAPDcs; DNAPK; DNA-PK; DNAPKc; DNA-PKC; DNA-PKcs; DNPK1; DOXNPH; dxnph; HYRC; HYRC1; IMD26; p350; slip; XRCC7
Chromosome: 16
Strain of origin: C.BKa-Igh^b/Icr
Site of expression: T and B lymphocytes.
Molecular note: A T-to-A transversion point mutation at a position corresponding to codon 4046 (codon 4095 in transcript ENSMUST00000023352.8) created a premature stop codon (p.Y4046*).