These MMTV-TGFα mice express a transgene containing the normal allele of the human transforming growth factor alpha gene and adult females exhibit alveolar gland hyperplasia.
Dr. Robert J. Coffey, Vanderbilt University Medical Center
Mice are viable and fertile, although mice of the most extensively studied line lactate insufficiently to support the litter. Virgin transgenic mice show no mammary gland abnormalities prepubertally, but adult virgin mice have considerable alveolar gland hyperplasia. Pregnant transgenic mice show marked proliferation of the stromal cells, and alveolar secretion is markedly increased compared to nontransgnic mice. After multiple pregnancies, isolated adenocarcinomas develop. There is no apparent phenotypic effect in males. In females, transgene expression is localized to the small ducts and alveoli in both virgin and pregnant mice as evidenced by in situ hybridization and by immunohistochemistry. Immunostaining also reveals some stromal staining in the hyperplastic areas. Egfr mRNA expression is also increased in mammary tissues expressing high levels of the transgene. Crosses between TGFA transgenic mice and TGFB1 transgenic mice demonstrate that these growth factors oppose each other's action on mammary gland development. The TGFA transgenic mice may also be useful in crosses with mice carrying mutations in the EGF and EGFR families. The putative tumor-promoter effect of TGFA may also be useful in enhancing the detection of tumor-initiating events and in determining the actions of putative metastases-inducing genes.
|Expressed Gene||TGFA, transforming growth factor alpha, human|
|Site of Expression|
|Allele Name||transgene insertion 29, Robert J Coffey|
|Allele Type||Transgenic (Inserted expressed sequence, Humanized sequence)|
|Gene Symbol and Name||Tg(MMTVTGFA)29Rjc, transgene insertion 29, Robert J Coffey|
|Promoter||MMTV, Mouse Mammary Tumor Virus, MMTV|
|Expressed Gene||TGFA, transforming growth factor alpha, human|
|Strain of Origin||(C57BL/6 x DBA/2)F2|
|Molecular Note||This transgene contains the mouse mammary tumor virus LTR and the normal allele of the human transforming growth factor alpha gene. The vector also contains exons 2 and 3 of the rabbit beta-globin gene. Lines 254, 257, 64 and 29 were produced.|
|Mutations Made By|| |
Dr. Robert Coffey, Vanderbilt University Medical Center
When using the MMTV-TGFα mouse strain in a publication, please cite the originating article(s) and include JAX stock #002373 in your Materials and Methods section.
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