These Zfy-1/lacZ mice express a transgene containing mouse Zfy1 fused with LacZ.
Dr. Ralph Brinster, University of Pennsylvania
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter) |
Transgenic mice show a strong expression of the lacZ gene in round spermatids and subsequent stages of spermatogenesis. These mice can be used as a source of germ cells and transplanted into busulfan treated mice (busulfan (40 mg/kg i.p.) destroys spermatogenic stem cells). LacZ serves as a reporter for the transplanted cells. Following transplantation, spermatogonia with stem cell potential establish themselves on the basal membrane of the recipient seminiferous tubule and begin to replicate. This strain has a higher copy number of the transgene than the TgN(c177lacZ)227Bri strain and may be distinguished from it by a dot blot. Cells from these two lines may therefore be mixed prior to injection and be distinguished from each other in the progeny.
This transgenic strain was made in the laboratory of Dr. Ralph Brinster of the University of Pennsylvania School of Veterinary Medicine. The transgene construct consisted of the lac-Z gene in association with an autosomal Zfy-like promoter. Per Elizabeth Simpson the promoter was Syx4 (similar sequence on Y and X 4). The genetic background is a mixed C57BL/6J x SJL.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression | Round spermatids and subsequent stages of spermatogenesis. |
Allele Name | transgene insertion 226, Ralph L Brinster |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | |
Gene Symbol and Name | Tg(c177-lacZ)226Bri, transgene insertion 226, Ralph L Brinster |
Gene Synonym(s) | |
Promoter | Zyf2, Zyf2, |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | Round spermatids and subsequent stages of spermatogenesis. |
Strain of Origin | Not Specified |
Chromosome | UN |
General Note | Line 226 has a higher transgene copy number than line 227. Transgenic mice show a strong expression of the lacZ gene in round spermatids and subsequent stages of spermatogenesis. |
Molecular Note | The transgene contains lacZ controlled by a Zyf2 promoter. Line 226 has a higher transgene copy number than line 227. |
This strain can be maintained by homozygous sibling matings. The genetic background is a mixed C57BL/6J x SJL. The expected coat color is black.
When using the Zfy-1/lacZ mouse strain in a publication, please cite the originating article(s) and include JAX stock #002369 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous for Tg(c177-lacZ)226Bri |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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