Transgenic mice show a strong expression of the <i>lacZ</i> gene in round spermatids and subsequent stages of spermatogenesis. These mice can be used as a source of germ cells and transplanted into busulfan treated mice (busulfan (40 mg/kg i.p.) destroys spermatogenic stem cells). <i>LacZ</i> serves as a reporter for the transplanted cells. Following transplantation, spermatogonia with stem cell potential establish themselves on the basal membrane of the recipient seminiferous tubule and begin to replicate. This strain has a higher copy number of the transgene than the TgN(c177lacZ)227Bri strain and may be distinguished from it by a dot blot. Cells from these two lines may therefore be mixed prior to injection and be distinguished from each other in the progeny.

These Zfy-1/lacZ mice express a transgene containing mouse Zfy1 fused with LacZ.

Typically mice are recovered in 12-16 weeks. Contact Customer Service to place an order or for more information.

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