C57BL/6J-Tg(pPWL512hyg)1Ems/J

Stock number: 002354
Product name: hyg^R
Research areas: Research Tools

Detailed description

These transgenic mice express the hygromycin-resistance gene in a wide variety of tissues including muscle. Embryonic feeder layers derived from transgenic mice are resistant to up to 0.8mg/ml hygromycin B. Feeder layers from normal C57BL/6J mice are killed by 0.2mg/ml hygromycin B.

Allele

Symbol: Tg(pPWL512hyg)1Ems
Name: transgene insertion 1, Elizabeth M Simpson
MGI accession ID: MGI:3052969
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(pPWL512hyg)1Ems
Marker name: transgene insertion 1, Elizabeth M Simpson
Chromosome: UN
Strain of origin: C57BL/6J
Expressed genes: hyg,hygromycin resistance,bacteria
Molecular note: The transgene contains a PGK promoter and the bacterial hygromycin resistance gene.
General note: Transgene also called "feeder" and "hyg."

Transgenic mice on a C57BL/6 background express the hygromycin resistance gene in a wide variety of tissues including muscle. Embryonic feeder layers derived from these mice are resistant to up to 0.8mg/ml hygromycin B, whereas feeder layers from normal C57BL/6J mice are killed by 0.2mg/ml hygromycin B.

Mouse embryonic fibroblasts prepared from homozygous transgenic mice that are also homozygous for Tg(Hprt1)1Jae and Dnmt1^tm3Jae are resistant to concentrations of the drugs G418, 6TG, puromycin, and hygromycin well above those used normally for the selection of drug-resistant ES cells.