Mice homozygous for the Wt1tm1Jae targeted mutation die between embryonic days 13 and 15. They fail to develop a kidney or gonads. The hearts of homozygous mutant mice also fail to develop properly. Hearts are smaller than wildtype controls and possess a rounded apex. The right ventricular wall is thin and the left ventricle is reduced in size. There appears to be normal development of the aortic, pulmonary, mitral and tricuspid valves. Development of the diaphragm is also incomplete resulting in incomplete separation of the thoracic and abdominal cavities. Homozygous mutant lungs are also markedly smaller than wild type lungs. Heterozygous mice appear normal and show no tumor development (mice followed until 10 months of age).
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
This targeted mutant was made in the Laboratory of Dr. Rudolf Jaenisch at the Whitehead Institute for Biomedical Research. A replacement-type targeting vector was used which resulted in the deletion of the first exon and 0.5 kb of upstream sequence. Targeting was done in J1 ES cells. This strain was produced by breeding the Wt1tm1Jae mutation to C57BL/6J mice (Stock No. 000664).
|Allele Name||targeted mutation 1, Rudolf Jaenisch|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Wt1, Wilms tumor 1 homolog|
|Strain of Origin||129S4/SvJae|
|Molecular Note||Exon 1 and 0.5kb of upstream sequence were replaced with a neomycin selection cassette.|
|Mutations Made By|| |
Rudolf Jaenisch, Whitehead Institute, Massachusetts Institute of Technology
The B6;129-Wt1tm1Jae strain is maintained by mating heterozygous carriers with wild type mice. Heterozygous breeder pairs are supplied, homozygotes die between embryonic days 13 and 15.
When using the B6;129S4-Wt1tm1Jae/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #002332 in your Materials and Methods section.