| Allele Name | transgene insertion 25, Walter and Eliza Hall Institute of Medical Research |
| Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
| Allele Synonym(s) | 25Wehi; Bcl2/Wehi25 Tg; Emu-2-25; Emu-bcl-2-25; H2k-Bcl2-tg; hBcl-2 |
| Gene Symbol and Name | Tg(BCL2)25Wehi, transgene insertion 25, Walter and Eliza Hall Institute of Medical Research |
| Gene Synonym(s) | |
| Promoter | SV40, E mu enhancer, SV40 |
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| Expressed Gene | BCL2, BCL2, apoptosis regulator, human |
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| Strain of Origin | (C57BL/6JWehi x SJL/JWehi)F2 |
| Chromosome | UN |
| General Note | In transgenic mice on a C57BL/6 background, expression of the transgene is restricted to the T cell lineage (no B-cell expression). Thymocytes, peripheral T-cells and activated T cells from these mice withstand prolonged culture in the absence of growth factors. Cells are resistant to killing by gamma-radiation, glucocorticoids, ionomycin, PMA, and sodium azide, but not complement, cytotoxic T cells or Fas ligand. Hemizygotes have a normal total T-cell count and thymic involution rate but show an enhanced response to immunization. This transgenic line displays no detectable autoimmunity. These mice serve as a robust source for the production of T-cell lines or hybridomas. |
| Molecular Note | The transgene construct consists of the human BCL2 cDNA in association with the Emu immunoglobulin heavy chain enhancer and SV40 promoter. |
| Mutations Made By | Dr. Alan Harris, The Walter & Eliza Hall Inst of Med Res |
