B6.Cg-Tg(BCL2)25Wehi/J

Stock number: 002320
Product name: bcl-2-25
Research areas: Apoptosis Research, Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

These bcl-2-25 mice express a transgene containing human B-cell lymphoma 2 (BCL2) cDNA that is restricted to the T cell lineage with no B-cell expression, and they display no detectable autoimmunity. They can be used for the production of T-cell lines or hybridomas.

Detailed description

Expression of the human BCL2 transgene restricted to the T cell lineage (no B-cell expression). Thymocytes, peripheral T-cells and activated T cells from these mice withstand prolonged culture in the absence of growth factors. Cells are resistant to killing by gamma-radiation, glucocorticoids, ionomycin, PMA and sodium azide but NOT complement, cytotoxic T cells or Fas ligand. Hemizygotes have a normal total T-cell count and thymic involution rate but show an enhanced response to immunization. This transgenic line displays no detectable autoimmunity. These mice serve as a robust source for the production of T-cell lines or hybridomas. Also known as 25Wehi or Emu-bcl-2-25.

Development

The TgN(BCL2)25Wehi transgenic strain was made in the laboratory of Dr. Alan Harris of the Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia. The transgene construct consisted of the human BCL2 cDNA in association with the Emu immunoglobulin heavy chain enhancer and SV40 promoter. Original background was mix of C57BL/6JWehi and SJL/JWehi. Transgenic mice have been bred to C57BL/6J more than 20 generations.

Allele

Symbol: Tg(BCL2)25Wehi
Name: transgene insertion 25, Walter and Eliza Hall Institute of Medical Research
MGI accession ID: MGI:3052945
Generation method: Transgenic
Attributes: Inserted expressed sequence; Humanized sequence
Marker symbol: Tg(BCL2)25Wehi
Marker name: transgene insertion 25, Walter and Eliza Hall Institute of Medical Research
Chromosome: UN
Strain of origin: (C57BL/6JWehi x SJL/JWehi)F2
Expressed genes: BCL2,BCL2, apoptosis regulator,human
Molecular note: The transgene construct consists of the human BCL2 cDNA in association with the Emu immunoglobulin heavy chain enhancer and SV40 promoter.
General note:

In transgenic mice on a C57BL/6 background, expression of the transgene is restricted to the T cell lineage (no B-cell expression).

Thymocytes, peripheral T-cells and activated T cells from these mice withstand prolonged culture in the absence of growth factors. Cells are resistant to killing by gamma-radiation, glucocorticoids, ionomycin, PMA, and sodium azide, but not complement, cytotoxic T cells or Fas ligand. Hemizygotes have a normal total T-cell count and thymic involution rate but show an enhanced response to immunization. This transgenic line displays no detectable autoimmunity. These mice serve as a robust source for the production of T-cell lines or hybridomas.