These bcl-2-25 mice express a transgene containing human B-cell lymphoma 2 (BCL2) cDNA that is restricted to the T cell lineage with no B-cell expression, and they display no detectable autoimmunity. They can be used for the production of T-cell lines or hybridomas.
Dr. Alan Harris, The Walter & Eliza Hall Inst of Med Res
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence, Humanized sequence) |
Expression of the human BCL2 transgene restricted to the T cell lineage (no B-cell expression). Thymocytes, peripheral T-cells and activated T cells from these mice withstand prolonged culture in the absence of growth factors. Cells are resistant to killing by gamma-radiation, glucocorticoids, ionomycin, PMA and sodium azide but NOT complement, cytotoxic T cells or Fas ligand. Hemizygotes have a normal total T-cell count and thymic involution rate but show an enhanced response to immunization. This transgenic line displays no detectable autoimmunity. These mice serve as a robust source for the production of T-cell lines or hybridomas. Also known as 25Wehi or Emu-bcl-2-25.
The TgN(BCL2)25Wehi transgenic strain was made in the laboratory of Dr. Alan Harris of the Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia. The transgene construct consisted of the human BCL2 cDNA in association with the Emu immunoglobulin heavy chain enhancer and SV40 promoter. Original background was mix of C57BL/6JWehi and SJL/JWehi. Transgenic mice have been bred to C57BL/6J more than 20 generations.
Expressed Gene | BCL2, BCL2, apoptosis regulator, human |
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Site of Expression |
Allele Name | transgene insertion 25, Walter and Eliza Hall Institute of Medical Research |
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Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | 25Wehi; Bcl2/Wehi25 Tg; Emu-2-25; Emu-bcl-2-25; H2k-Bcl2-tg; hBcl-2 |
Gene Symbol and Name | Tg(BCL2)25Wehi, transgene insertion 25, Walter and Eliza Hall Institute of Medical Research |
Gene Synonym(s) | |
Promoter | SV40, E mu enhancer, SV40 |
Expressed Gene | BCL2, BCL2, apoptosis regulator, human |
Strain of Origin | (C57BL/6JWehi x SJL/JWehi)F2 |
Chromosome | UN |
General Note | In transgenic mice on a C57BL/6 background, expression of the transgene is restricted to the T cell lineage (no B-cell expression). Thymocytes, peripheral T-cells and activated T cells from these mice withstand prolonged culture in the absence of growth factors. Cells are resistant to killing by gamma-radiation, glucocorticoids, ionomycin, PMA, and sodium azide, but not complement, cytotoxic T cells or Fas ligand. Hemizygotes have a normal total T-cell count and thymic involution rate but show an enhanced response to immunization. This transgenic line displays no detectable autoimmunity. These mice serve as a robust source for the production of T-cell lines or hybridomas. |
Molecular Note | The transgene construct consists of the human BCL2 cDNA in association with the Emu immunoglobulin heavy chain enhancer and SV40 promoter. |
Mutations Made By | Dr. Alan Harris, The Walter & Eliza Hall Inst of Med Res |
This strain is maintained by mating hemizygous mice with wildtype siblings. Expected coat color from breeding:Black
When using the bcl-2-25 mouse strain in a publication, please cite the originating article(s) and include JAX stock #002320 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(BCL2)25Wehi |
Frozen Mouse Embryo | B6.Cg-Tg(BCL2)25Wehi/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(BCL2)25Wehi/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(BCL2)25Wehi/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(BCL2)25Wehi/J Frozen Embryos | $3373.50 |
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