These Il7r knock-out mice exhibit reduced thymic and splenic lymphoid cellularity in both B and T cell compartments.
Dr. Jacques Peschon, Amgen
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Il7r | interleukin 7 receptor |
Mice homozygous for the Il7rtm1Imx targeted mutation are viable and fertile. Newborn and young adult homozygous mutant mice have dramatically reduced thymic and splenic lymphoid cellularity in both B and T cell compartments. T cell development is affected prior to surface expression of CD4 and CD8 and prior to the initiation of T cell receptor beta chain rearrangement. B cell development is through pre-pro-B cells (Cd43+ HSAdull). The phenotype in the mouse resembles human interleukin-2 receptor gamma deficiency (XSCID). IL2Rgamma has been shown to be a necessary component of the IL7R providing the biochemical basis for this condition.
Allele Name | targeted mutation 1, Immunex |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | CD127-; IL-7R-; IL7R-; IL-7Ralpha- |
Gene Symbol and Name | Il7r, interleukin 7 receptor |
Gene Synonym(s) | |
Strain of Origin | 129S7/SvEvBrd-Hprt+ |
Chromosome | 15 |
Molecular Note | A neomycin resistance cassette was inserted into exon 3 of the gene. |
Mutations Made By | Dr. Jacques Peschon, Amgen |
The Il7r deficient strain is maintained by homozygous sibling matings. This strain should be housed under pathogen free conditions similar to the Prkdcscid/Prkdcscid mouse or any other immunodeficient strain. The expected coat color from breeding is black or white-bellied agouti.
When using the IL-7R- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002296 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Il7r<tm1Imx> |
Frozen Mouse Embryo | B6;129S7-Il7r<tm1Imx>/J | $2595.00 |
Frozen Mouse Embryo | B6;129S7-Il7r<tm1Imx>/J | $2595.00 |
Frozen Mouse Embryo | B6;129S7-Il7r<tm1Imx>/J | $3373.50 |
Frozen Mouse Embryo | B6;129S7-Il7r<tm1Imx>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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