B6;129S7-Il7r^tm1Imx/J

Stock number: 002296
Product name: IL-7R-
Research areas: Cancer Research, Immunology, Inflammation and Autoimmunity Research

Description

These Il7r knock-out mice exhibit reduced thymic and splenic lymphoid cellularity in both B and T cell compartments.

Detailed description

Mice homozygous for the Il7r^tm1Imx targeted mutation are viable and fertile. Newborn and young adult homozygous mutant mice have dramatically reduced thymic and splenic lymphoid cellularity in both B and T cell compartments. T cell development is affected prior to surface expression of CD4 and CD8 and prior to the initiation of T cell receptor beta chain rearrangement. B cell development is through pre-pro-B cells (Cd43⁺ HSA^dull). The phenotype in the mouse resembles human interleukin-2 receptor gamma deficiency (XSCID). IL2Rgamma has been shown to be a necessary component of the IL7R providing the biochemical basis for this condition.

Allele

Symbol: Il7r^tm1Imx
Name: targeted mutation 1, Immunex
MGI accession ID: MGI:1857198
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Il7r
Marker name: interleukin 7 receptor
Chromosome: 15
Strain of origin: 129S7/SvEvBrd-Hprt⁺
Molecular note: A neomycin resistance cassette was inserted into exon 3 of the gene.