Overview

Also Known As: P-selectin^-

Mice homozygous for Selp^tm1Bay show a deficit in leukocyte rolling and delayed neutrophil extravasation in response to intraperitoneal injections of thioglycollate. The strain can be used as model for human leukocyte adhesion deficiency I syndrome.

Donating Investigator

Dr. Arthur Beaudet, Baylor College of Medicine

Genetic overview

Genetic Background	Generation
000664 C57BL/6J	N10F59 (2019-06-14 00:00:00)

Selp^tm1Bay

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Selp	selectin, platelet

View Genetics

Research Applications

Immunology, Inflammation and Autoimmunity Research

View All Research Applications

Base Price

Starting at:

$278.00 Domestic price for female

View Price List

Details

Detailed Description

Mice homozygous for the Selp^tm1Bay mutation are viable and fertile. Homoyzgous mutant mice show a deficit in leukocyte rolling and delayed neutrophil extravasation in response to intraperitoneal injections of thioglycollate. Neutrophil count is elevated (2-3-fold). Circulating lymphocyte and monocyte counts are normal. 60-70% reduction in neutrophil emigration into the peritoneum during Streptococcus pneumoniae-induced peritonitis is reduced 60-70%. It can be used as model for human leukocyte adhesion deficiency I syndrome (characterized by elevated levels of circulating leukocytes and impaired leukocyte extravasation to sites of inflammation or infection).

Development

A neomycin resistance gene was inserted in the reverse orientation to delete a 4.5kb region containing exons 3 through 5. RT-PCR analysis of lung RNA isolated from homozygous mutant mice showed a transcript 682 base pairs shorter than normal. A clone with the correctly targeted gene was injected into C57BL/6 embryos. The strain has been backcrossed for 10 generations to C57BL/6J.

Control Suggestions

Approximate Controls

Additional Information

Considerations for Choosing Controls

Selected References

Bullard DC; Qin L; Lorenzo I; Quinlin WM; Doyle NA; Bosse R; Vestweber D; Doerschuk CM; Beaudet AL. 1995. P-selectin/ICAM-1 double mutant mice: acute emigration of neutrophils into the peritoneum is completely absent but is normal into pulmonary alveoli [see comments] J Clin Invest 95(4):1782-8PubMed: 7535798 MGI: J:24239

View All References

Genetics

Selp^tm1Bay

Allele Symbol: Selp^tm1Bay

Allele Name	targeted mutation 1, Baylor College of Medicine
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	targeted mutation 1, Baylor College of Medicine; Selp^tm1Bay
Gene Symbol and Name	Selp, selectin, platelet
Gene Synonym(s)	CD62; CD62P; PSEL; GRMP; granulocyte membrane protein; LECAM3; Grmp; P-selectin; PADGEM; GMP140; PSELECT; Grmp
Strain of Origin	129S7/SvEvBrd-Hprt^b-m2
Chromosome	1
Molecular Note	A neomycin resistance gene was inserted in the reverse orientation to delete a 4.5kb region containing exons 3 through 5. RT-PCR analysis of lung RNA isolated from homozygous mutant mice showed a transcript 682 base pairs shorter than normal. Immunohistochemical staining showed a lack of encoded protein on both endothelium and platelets.
Mutations Made By	Dr. Arthur Beaudet, Baylor College of Medicine

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: Selp^tm1Bay related

Immunology, Inflammation and Autoimmunity Research
- CD Antigens, Antigen Receptors, and Histocompatibility Markers
- Inflammation

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Selp^tm1Bay/Selp^tm1Bay
involves: 129S7/SvEvBrd * C57BL/6J

behavior/neurological phenotype

abnormal pilomotor reflex
- observed following infection with S. pneumoniae

head tilt
- observed following infection with S. pneumoniae

hypoactivity
- observed following infection with S. pneumoniae

spinning
- observed following infection with S. pneumoniae

tremors
- observed following infection with S. pneumoniae

immune system phenotype

abnormal leukocyte migration
- Streptococcus pneumoniae induced peritonitis reduced neutrophil emigration into peritoneum by 61-69% after 2-4 hours

abnormal spleen morphology
- increase in splenic thrombosis and necrosis in contrast to controls following infection with S. pneumoniae

eye inflammation
- panophthalmitis observed in 8% of mice following infection with S. pneumoniae

increased leukocyte cell number
- elevated peripheral leukocyte count in contrast to controls following infection with S. pneumoniae

increased neutrophil cell number
- 2-3 fold increase in numbers of circulating neutrophils
- mild neutrophilia in contrast to controls following infection with S. pneumoniae

increased susceptibility to bacterial infection
- decreased ability to clear bacteremia among survivors
- severity and duration of morbidity higher than wild-type after infection with Streptococcus pneumoniae
- survival rate was 19% in contrast to a 57% rate for wild-type mice

liver/biliary system phenotype

hepatic necrosis
- higher incidence in contrast to controls following infection with S. pneumoniae

vision/eye phenotype

eye inflammation
- panophthalmitis observed in 8% of mice following infection with S. pneumoniae

cellular phenotype

abnormal leukocyte migration
- Streptococcus pneumoniae induced peritonitis reduced neutrophil emigration into peritoneum by 61-69% after 2-4 hours

hepatic necrosis
- higher incidence in contrast to controls following infection with S. pneumoniae

growth/size/body region phenotype

weight loss
- observed 48 hours post-infection with S. pneumoniae

hematopoietic system phenotype

abnormal leukocyte migration
- Streptococcus pneumoniae induced peritonitis reduced neutrophil emigration into peritoneum by 61-69% after 2-4 hours

abnormal spleen morphology
- increase in splenic thrombosis and necrosis in contrast to controls following infection with S. pneumoniae

increased leukocyte cell number
- elevated peripheral leukocyte count in contrast to controls following infection with S. pneumoniae

increased neutrophil cell number
- 2-3 fold increase in numbers of circulating neutrophils
- mild neutrophilia in contrast to controls following infection with S. pneumoniae

Genotype: Selp^tm1Bay/Selp^tm1Bay
D1.129S7(B6J)-Selp

hematopoietic system phenotype

increased IgG level
- increased levels of serum IgG anti-type II collagen antibody measured in collagen immunized mice as compared to immunized DBA/1 mice

immune system phenotype

abnormal cytokine secretion
- decreased levels of IL2 and IL4 measured in stimulated splenocytes
- increased levels of IL10 and IL5 measured in stimulated splenocytes

increased IgG level
- increased levels of serum IgG anti-type II collagen antibody measured in collagen immunized mice as compared to immunized DBA/1 mice

increased susceptibility to induced arthritis
- accelerated incidence of induced arthritis, 23% compared to 2% of DBA/1 mice by 28 days
- consistently higher arthritis severity scores as determined by inflammation, paw/digit distortion and ankylosis
- increase in number of affected paws
- onset of arthritis occurs as early as day 14 with a median onset of 34 days

skeleton phenotype

increased susceptibility to induced arthritis
- accelerated incidence of induced arthritis, 23% compared to 2% of DBA/1 mice by 28 days
- consistently higher arthritis severity scores as determined by inflammation, paw/digit distortion and ankylosis
- increase in number of affected paws
- onset of arthritis occurs as early as day 14 with a median onset of 34 days

Genotype: Selp^tm1Bay/Selp^tm1Bay
involves: 129S7/SvEvBrd * C57BL/6

hematopoietic system phenotype

increased neutrophil cell number
- circulating neutrophils are increased in mutants and this increases with age of mice

immune system phenotype

increased neutrophil cell number
- circulating neutrophils are increased in mutants and this increases with age of mice

The following phenotype relates to a compound genotype created using this strain

Genotype: Selp^tm1Bay/Selp^tm1Bay
B6.129S7-Selp/J

hematopoietic system phenotype

abnormal T cell physiology
- T lymphocyte migration and accumulation into cutaneous inflammatory regions induced by a combination of IFN-gamma and TNF-alpha is inhibited by about 25%, however IFN-gamma, TNF-alpha, and ConA, has no effect on T cell infiltration

immune system phenotype

abnormal T cell physiology
- T lymphocyte migration and accumulation into cutaneous inflammatory regions induced by a combination of IFN-gamma and TNF-alpha is inhibited by about 25%, however IFN-gamma, TNF-alpha, and ConA, has no effect on T cell infiltration

Genotype: Selp^tm1Bay/Selp^tm1Bay
either: B6.129S7-Selp or (involves: 129S7/SvEvBrd * C57BL/6)

cellular phenotype

abnormal leukocyte tethering or rolling
- after surgery to exteriorize cremaster muscle, mice do not display leukocyte rolling for the first hour, but a significant amount of rolling is detected after 2 hours (~8% rolling leukocyte flux)
- leukocytes show intermittent interactions with endothelium causing an increase in rolling velocity to ~129 um/second (~35 um/second in wild-type)
- treatment with Tnfa (tumor necrosis factor alpha) reduces rolling velocity to ~5 um/second

hematopoietic system phenotype

abnormal leukocyte tethering or rolling
- after surgery to exteriorize cremaster muscle, mice do not display leukocyte rolling for the first hour, but a significant amount of rolling is detected after 2 hours (~8% rolling leukocyte flux)
- leukocytes show intermittent interactions with endothelium causing an increase in rolling velocity to ~129 um/second (~35 um/second in wild-type)
- treatment with Tnfa (tumor necrosis factor alpha) reduces rolling velocity to ~5 um/second

increased leukocyte cell number
- systemic leukocyte counts are ~9800/ul (~61% neutrophils, ~37% lymphocytes) compared to wild-type counts of ~5660/ul (53% neutrophils, 46% lymphocytes)

immune system phenotype

abnormal leukocyte tethering or rolling
- after surgery to exteriorize cremaster muscle, mice do not display leukocyte rolling for the first hour, but a significant amount of rolling is detected after 2 hours (~8% rolling leukocyte flux)
- leukocytes show intermittent interactions with endothelium causing an increase in rolling velocity to ~129 um/second (~35 um/second in wild-type)
- treatment with Tnfa (tumor necrosis factor alpha) reduces rolling velocity to ~5 um/second

increased leukocyte cell number
- systemic leukocyte counts are ~9800/ul (~61% neutrophils, ~37% lymphocytes) compared to wild-type counts of ~5660/ul (53% neutrophils, 46% lymphocytes)

Genotype: Selp^tm1Bay/Selp^tm1Bay
B6.129S7-Selp

hematopoietic system phenotype

decreased mast cell number
- during the chronic phase of antigen exposure

immune system phenotype

decreased mast cell number
- during the chronic phase of antigen exposure

decreased susceptibility to type IV hypersensitivity reaction
- chronic inflammation in response to oxazolone exposure is inhibited compared to in similarly treated wild-type mice
- Normal - however, acute inflammation in response to oxazolone exposure is normal

References

Bullard DC; Qin L; Lorenzo I; Quinlin WM; Doyle NA; Bosse R; Vestweber D; Doerschuk CM; Beaudet AL. 1995. P-selectin/ICAM-1 double mutant mice: acute emigration of neutrophils into the peritoneum is completely absent but is normal into pulmonary alveoli [see comments] J Clin Invest 95(4):1782-8PubMed: 7535798 MGI: J:24239

Additional References

Bullard DC; Kunkel EJ; Kubo H; Hicks MJ; Lorenzo I; Doyle NA; Doerschuk CM; Ley K; Beaudet AL. 1996. Infectious susceptibility and severe deficiency of leukocyte rolling and recruitment in E-selectin and P-selectin double mutant mice. J Exp Med 183(5):2329-36PubMed: 8642341 MGI: J:33135
Doerschuk CM; Quinlan WM; Doyle NA; Bullard DC; Vestweber D; Jones ML; Takei F; Ward PA; Beaudet AL. 1996. The role of P-selectin and ICAM-1 in acute lung injury as determined using blocking antibodies and mutant mice. J Immunol 157(10):4609-14PubMed: 8906840 MGI: J:37197
Jung U; Ley K. 1999. Mice lacking two or all three selectins demonstrate overlapping and distinct functions for each selectin. J Immunol 162(11):6755-62PubMed: 10352295 MGI: J:55032
Kamochi M; Kamochi F; Kim YB; Sawh S; Sanders JM; Sarembock I; Green S; Young JS; Ley K; Fu SM; Rose CE Jr. 1999. P-selectin and ICAM-1 mediate endotoxin-induced neutrophil recruitment and injury to the lung and liver. Am J Physiol 277(2 Pt 1):L310-9PubMed: 10444525 MGI: J:56844
Bullard DC; Mobley JM; Justen JM; Sly LM; Chosay JG; Dunn CJ; Lindsey JR; Beaudet AL; Staite ND. 1999. Acceleration and increased severity of collagen-induced arthritis in P-selectin mutant mice. J Immunol 163(5):2844-9PubMed: 10453030 MGI: J:57086
Tesfaigzi Y; Fischer MJ; Daheshia M; Green FH; De Sanctis GT; Wilder JA. 2002. Bax is crucial for IFN-gamma-induced resolution of allergen-induced mucus cell metaplasia. J Immunol 169(10):5919-25PubMed: 12421976 MGI: J:80074
Huo Y; Schober A; Forlow SB; Smith DF; Hyman MC; Jung S; Littman DR; Weber C; Ley K. 2003. Circulating activated platelets exacerbate atherosclerosis in mice deficient in apolipoprotein E. Nat Med 9(1):61-7PubMed: 12483207 MGI: J:81164
Sun G; Chang WL; Li J; Berney SM; Kimpel D; van der Heyde HC. 2003. Inhibition of platelet adherence to brain microvasculature protects against severe Plasmodium berghei malaria. Infect Immun 71(11):6553-61PubMed: 14573677 MGI: J:86276
Kohm AP; Miller SD. 2003. Role of ICAM-1 and P-selectin expression in the development and effector function of CD4+CD25+regulatory T cells. J Autoimmun 21(3):261-71PubMed: 14599851 MGI: J:86435

Additional - Selp^tm1Bay related

Technical Support

Contact Technical Support

Genotyping Protocols
- Standard PCR:Selp^tm1Bay
- Standard PCR:Selp^tm1Bay
- Genotyping resources and troubleshooting
Dietary Information
- LabDiet® 5K52 formulation (6% fat)
Mating System
- Homozygote x Homozygote
Appearance
- black
  Related Genotype: a/a
Citation
When using the P-selectin^- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002289 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX11 (Maximum)

Pricing & Availability

Available

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Homozygous for Selp<tm1Bay>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

Terms Of Use

Terms of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Use of MICE by companies or for-profit entities requires a license prior to shipping.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED "AS IS". JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

Credit for PRODUCTS or SERVICES

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of, PRODUCTS or SERVICES, JACKSON will, at its option, provide credit or replacement for the PRODUCT received or the SERVICES provided; JACKSON makes no other representations and this shall be the exclusive remedy of the purchaser. Please note specific policy for live mice.

Animal Care and Use for SERVICES

Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.

Also Known As: P-selectin-

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Approximate Controls

Additional Information

Selected References

Selptm1Bay

Allele Symbol: Selptm1Bay

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: Selptm1Bay related

Mammalian Phenotype Terms by Genotype

Genotype: Selptm1Bay/Selptm1Bayinvolves: 129S7/SvEvBrd * C57BL/6J

behavior/neurological phenotype

immune system phenotype

liver/biliary system phenotype

vision/eye phenotype

cellular phenotype

growth/size/body region phenotype

hematopoietic system phenotype

Genotype: Selptm1Bay/Selptm1BayD1.129S7(B6J)-Selp

hematopoietic system phenotype

immune system phenotype

skeleton phenotype

Genotype: Selptm1Bay/Selptm1Bayinvolves: 129S7/SvEvBrd * C57BL/6

hematopoietic system phenotype

immune system phenotype

Genotype: Selptm1Bay/Selptm1BayB6.129S7-Selp/J

hematopoietic system phenotype

immune system phenotype

Genotype: Selptm1Bay/Selptm1Bayeither: B6.129S7-Selp or (involves: 129S7/SvEvBrd * C57BL/6)

cellular phenotype

hematopoietic system phenotype

immune system phenotype

Genotype: Selptm1Bay/Selptm1BayB6.129S7-Selp

hematopoietic system phenotype

immune system phenotype

References

Additional References

Additional - Selptm1Bay related

Genotyping Protocols

Dietary Information

Mating System

Appearance

Citation

Animal Health Reports

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Also Known As: P-selectin^-

Selp^tm1Bay

Allele Symbol: Selp^tm1Bay

Genotype: Selp^tm1Bay related

Genotype: Selp^tm1Bay/Selp^tm1Bay
involves: 129S7/SvEvBrd * C57BL/6J

Genotype: Selp^tm1Bay/Selp^tm1Bay
D1.129S7(B6J)-Selp

Genotype: Selp^tm1Bay/Selp^tm1Bay
involves: 129S7/SvEvBrd * C57BL/6

Genotype: Selp^tm1Bay/Selp^tm1Bay
B6.129S7-Selp/J

Genotype: Selp^tm1Bay/Selp^tm1Bay
either: B6.129S7-Selp or (involves: 129S7/SvEvBrd * C57BL/6)

Genotype: Selp^tm1Bay/Selp^tm1Bay
B6.129S7-Selp

Additional - Selp^tm1Bay related