Mice homozygous for Selptm1Bay show a deficit in leukocyte rolling and delayed neutrophil extravasation in response to intraperitoneal injections of thioglycollate. The strain can be used as model for human leukocyte adhesion deficiency I syndrome.
Dr. Arthur Beaudet, Baylor College of Medicine
Genetic Background | Generation |
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000664 C57BL/6J |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Selp | selectin, platelet |
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Mice homozygous for the Selptm1Bay mutation are viable and fertile. Homoyzgous mutant mice show a deficit in leukocyte rolling and delayed neutrophil extravasation in response to intraperitoneal injections of thioglycollate. Neutrophil count is elevated (2-3-fold). Circulating lymphocyte and monocyte counts are normal. 60-70% reduction in neutrophil emigration into the peritoneum during Streptococcus pneumoniae-induced peritonitis is reduced 60-70%. It can be used as model for human leukocyte adhesion deficiency I syndrome (characterized by elevated levels of circulating leukocytes and impaired leukocyte extravasation to sites of inflammation or infection).
A neomycin resistance gene was inserted in the reverse orientation to delete a 4.5kb region containing exons 3 through 5. RT-PCR analysis of lung RNA isolated from homozygous mutant mice showed a transcript 682 base pairs shorter than normal. A clone with the correctly targeted gene was injected into C57BL/6 embryos. The strain has been backcrossed for 10 generations to C57BL/6J.
Allele Name | targeted mutation 1, Baylor College of Medicine |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | CD62ELP-; CD62P-; P-; P-sel-; P-selectin- |
Gene Symbol and Name | Selp, selectin, platelet |
Gene Synonym(s) | |
Strain of Origin | 129S7/SvEvBrd-Hprtb-m2 |
Chromosome | 1 |
Molecular Note | A neomycin resistance gene was inserted in the reverse orientation to delete a 4.5kb region containing exons 3 through 5. RT-PCR analysis of lung RNA isolated from homozygous mutant mice showed a transcript 682 base pairs shorter than normal. Immunohistochemical staining showed a lack of encoded protein on both endothelium and platelets. |
Mutations Made By | Dr. Arthur Beaudet, Baylor College of Medicine |
When using the P-selectin- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002289 in your Materials and Methods section.
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Service/Product | Description | Price |
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Homozygous for Selp<tm1Bay> |
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