B6.129S7-Ifng^tm1Ts/J

Stock number: 002287
Product name: Ifng KO
Research areas: Cancer Research, Hematological Research, Immunology, Inflammation and Autoimmunity Research

Description

Macrophages from mice homozygous for the Ifng^tm1Ts knock-out allele show impaired production of antimicrobial products and reduced expression of MHC II antigens. There is also uncontrollable proliferation of splenocytes from homozygotes in response to mitogens and alloantigens and a reduced resting natural killer cell activity.

Detailed description

Mice homozygous for the Ifng^tm1Ts targeted mutation are viable and fertile. Homozygotes appear normal in a "clean" environment but display reduced macrophage function in response to pathogens. Specifically, macrophages show impaired production of antimicrobial products and reduced expression of MHC II antigens. There is also uncontrollable proliferation of splenocytes from homozygotes in response to mitogens and alloantigens and a reduced resting natural killer cell activity.

Development

A targeting vector containing a neo cassette was used to disrupt exon 2. The construct was electroporated into 129S7/SvEvBrd-Hprt1⁺ derived AB1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting mice were backcrossed onto the C57BL/6 background for 8 generations by the donating investigator before arriving at The Jackson Laboratory.

Allele

Symbol: Ifng^tm1Ts
Name: targeted mutation 1, Timothy Stewart
MGI accession ID: MGI:1857184
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Ifng
Marker name: interferon gamma
Chromosome: 10
Strain of origin: 129S7/SvEvBrd-Hprt⁺
Molecular note: Insertion of a neomycin gene into exon 2, which introduced a termination codon after the first 30 amino acids of the mature protein.