Mice homozygous for Ltatm1Dch show abnormal development of peripheral lymphoid organs with no detectable popliteal, inguinal, para-aortic, mesenteric, axillary, or cervical lymph nodes, and no detectable Peyer's patches.
IMR Colony, The Jackson Laboratory
Mice homozygous Ltatm1Dch targeted mutation are viable and fertile. Homozygous mutant mice show abnormal development of peripheral lymphoid organs with no detectable popliteal, inguinal, para-aortic, mesenteric, axillary, or cervical lymph nodes, and no detectable Peyer's patches. Morphological changes in the spleen white pulp were accompanied by alterations in T and B cell content. CD4+ and CD8+ T cells counts in peripheral blood are normal but there is a four-fold increase in B cells. Neutrophil, monocyte, and platelet counts are normal. The thymus contains normal numbers of CD4+CD8+, CD4+, CD8+, and CD4-CD8- T cells. Splenic T cells develop normal MHC class I and class II-restricted allocytotoxic responses. Also known as tumor necrosis factor beta, Tnfb.
The targeting strategy resulted in the elemination of exon 3 (4 exons total) and the introduction of a stop codon in exon 2. The predominant 1.5 and 1.8 kb Lta transcripts are missing and a major 3 kb transcript consistent with the structure of the targeted gene is present. 129 derived D3 ES cells were used for the targeting. The Ltatm1Dch targeted mutation was backcrossed to the C57BL/6J inbred strain for 5 generations (N5) prior to establishing a homozygous colony. Backcrossing is continuing to N10.
|Allele Name||targeted mutation 1, David D Chaplin|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||LT-; Lta-; LT-alpha-; LTalpha-|
|Gene Symbol and Name||Lta, lymphotoxin A|
|Strain of Origin||129S2/SvPas|
|Molecular Note||A neomycin resistance cassette replaced a small portion of exon 2, all of exon 3, and a small portion of exon 4.|
|Mutations Made By|| |
Dr. David Chaplin, University of Alabama at Birmingham
This strain is maintained by homozygous sibling matings. Expected coat color from breeding:Black
When using the LT- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002258 in your Materials and Methods section.