B6;129S2-Lta^tm1Dch/J

Stock number: 002257
Product name: LT^-
Research areas: Cancer Research, Immunology, Inflammation and Autoimmunity Research

Description

These Lta knock-out mice exhibit no morphologically detectable lymph nodes or Peyer's patches.

Detailed description

Mice homozygous Lta^tm1Dch targeted mutation are viable and fertile. Homozygous mutant mice show abnormal development of peripheral lymphoid organs with no detectable popliteal, inguinal, para-aortic, mesenteric, axillary, or cervical lymph nodes, and no detectable Peyer's patches. Morphological changes in the spleen white pulp were accompanied by alterations in T and B cell content. CD4⁺ and CD8⁺ T cells counts in peripheral blood are normal but there is a four-fold increase in B cells. Neutrophil, monocyte, and platelet counts are normal. The thymus contains normal numbers of CD4⁺CD8⁺, CD4⁺, CD8⁺, and CD4^-CD8^- T cells. Splenic T cells develop normal MHC class I and class II-restricted allocytotoxic responses. Also known as tumor necrosis factor beta, Tnfb.

Development

The targeting strategy resulted in the elemination of exon 3 (4 exons total) and the introduction of a stop codon in exon 2. The predominant 1.5 and 1.8 kb Lta transcripts are missing and a major 3 kb transcript consistent with the structure of the targeted gene is present. 129 derived D3 ES cells were used for the targeting

Allele

Symbol: Lta^tm1Dch
Name: targeted mutation 1, David D Chaplin
MGI accession ID: MGI:1857215
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Lta
Marker name: lymphotoxin A
Chromosome: 17
Strain of origin: 129S2/SvPas
Molecular note: A neomycin resistance cassette replaced a small portion of exon 2, all of exon 3, and a small portion of exon 4.