These Lta knock-out mice exhibit no morphologically detectable lymph nodes or Peyer's patches.
Dr. David Chaplin, University of Alabama at Birmingham
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Lta | lymphotoxin A |
Mice homozygous Ltatm1Dch targeted mutation are viable and fertile. Homozygous mutant mice show abnormal development of peripheral lymphoid organs with no detectable popliteal, inguinal, para-aortic, mesenteric, axillary, or cervical lymph nodes, and no detectable Peyer's patches. Morphological changes in the spleen white pulp were accompanied by alterations in T and B cell content. CD4+ and CD8+ T cells counts in peripheral blood are normal but there is a four-fold increase in B cells. Neutrophil, monocyte, and platelet counts are normal. The thymus contains normal numbers of CD4+CD8+, CD4+, CD8+, and CD4-CD8- T cells. Splenic T cells develop normal MHC class I and class II-restricted allocytotoxic responses. Also known as tumor necrosis factor beta, Tnfb.
The targeting strategy resulted in the elemination of exon 3 (4 exons total) and the introduction of a stop codon in exon 2. The predominant 1.5 and 1.8 kb Lta transcripts are missing and a major 3 kb transcript consistent with the structure of the targeted gene is present. 129 derived D3 ES cells were used for the targeting
Allele Name | targeted mutation 1, David D Chaplin |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | LT-; Lta-; LT-alpha-; LTalpha- |
Gene Symbol and Name | Lta, lymphotoxin A |
Gene Synonym(s) | |
Strain of Origin | 129S2/SvPas |
Chromosome | 17 |
Molecular Note | A neomycin resistance cassette replaced a small portion of exon 2, all of exon 3, and a small portion of exon 4. |
Mutations Made By | Dr. David Chaplin, University of Alabama at Birmingham |
This strain is currently maintained by homozygous sibling matings. Expected coat color from breeding:White Bellied Agouti and Black
When using the LT- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002257 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Homozygous for Lta<tm1Dch> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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