B6;129S2-Il6^tm1Kopf/J

Stock number: 002254
Product name: IL-6^-
Research areas: Cancer Research, Hematological Research, Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research, Research Tools

Description

These interleukin 6 (IL6) knock-out mutant mice show defects in responses to various viruses and in inflammatory responses to tissue damage or infection.

Detailed description

Mice homozygous for the targeted mutation are viable and fertile. No gene product (mRNA) is detected by RT-PCR analysis of lipopolysaccharide (LPS) challenged macrophages. Bioassay and enzyme-linked immunosorbent assay (ELISA) analysis of serum from LPS-challenged homozygotes reveals no detectable protein activity. These interleukin 6 (IL6) mutant mice show defects in responses to various viruses and in inflammatory responses to tissue damage or infection.

Of note, IL6-mutant mice may be available on different genetic backgrounds including mixed B6;129S2 (Stock No. 002254), C57BL/6J (Stock No. 002650), BALB/cByJ (Stock No. 007078), and NOD/ShiLtJ (Stock No. 029264).

Development

The interleukin 6 targeted mutation was created by Manfred Kopf and Georges Kohler (Max Planck Institut Fur Immunbiologie, Freiburg Germany). A targeting vector was designed to place a neomycin resistance cassette into the first coding exon (exon 2) of the targeted gene. This construct was electroporated into 129S2/SvPas-derived D3 embryonic stem (ES) cells. Heterozygotes were intercrossed to generate homozygotes.

Allele

Symbol: Il6^tm1Kopf
Name: targeted mutation 1, Manfred Kopf
MGI accession ID: MGI:1857197
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Il6
Marker name: interleukin 6
Chromosome: 5
Strain of origin: 129S2/SvPas
Molecular note: A neomycin selection cassette was inserted into exon 2. RT-PCR experiments on RNA derived from LPS-stimulated macrophages of homozygous mice demonstrated that no detectable transcript was produced from this allele. ELISA and bioassay experiments confirmed that no functional protein was made in homozygous mice.