Mice homozygous for the Il4tm1Cgn targeted mutation are viable and fertile. T and B cell development is normal but IgGl and IgE levels and the ability of homozygous mutant mice to produce Th2-derived cytokines are significantly reduced.
A targeting vector containing neomycin resistance and Herpes simplex virus thymidine kinase genes was used to disrupt Il4 exon 1. The construct was electroporated into 129P2/OlaHsd-derived E14-1 embryonic stem cells. The donating investigator reports that correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric animals were crossed to C57BL/6 mice (see SNP note below).
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 2 of 5 markers that determine C57BL/6J from C57BL/6N were found to be C57BL/6N. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
|Allele Name||targeted mutation 1, University of Cologne|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||IL-4-; IL-4 KO; IL4-; IL4tm1cgn129; IL-4KO; IL4T; IL-4T-|
|Gene Symbol and Name||Il4, interleukin 4|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||A translational stop codon and a neomycin resistance gene were inserted into the first exon of the gene.|
|Mutations Made By|| |
Dr. Ralf Kuhn, Max-Delbrück Center for Molecular Medicine
Expected coat color from breeding is Black.
When using the B6.129P2-Il4tm1Cgn/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #002253 in your Materials and Methods section.