cellular phenotype

• abnormal osteoblast physiology
  • osteocalcin level in sera are half that of wild-type mice indicating less osteoblast activity
  (MGI Ref ID J:107359)
  • percent of bone surface labeled in vivo with tetracycline is 32.2% versus 49.0% in wild-type controls
  (MGI Ref ID J:107359)
  • there is a profound decrease in alkaline phosphatase activity of osteoblasts lining the periosteum, endosteum, and trabeculae
  (MGI Ref ID J:107359)

hematopoietic system phenotype

• abnormal T cell differentiation
  • an increased proportion of CD3⁺ thymocytes co-express alphabeta TCR in the thymus of 6 week old mice
  (MGI Ref ID J:92453)

• abnormal osteoclast physiology
  • tartate-resistant acid phosphatase activity by osteoclasts is markedly reduced
  (MGI Ref ID J:107359)

• absent CD8-positive, alpha-beta T cells
  • CD8 T cells are absent in the spleen, lymph nodes, and in circulation
  (MGI Ref ID J:92453)

• decreased CD4-positive, alpha beta T cell number
  • there is a 5-fold drop in the number of CD4 T cells found in lymph nodes
  (MGI Ref ID J:92453)

• increased CD8-positive, alpha-beta T cell number
  • the proportion and absolute number of CD8 T cells is greatly increased in the thymus
  (MGI Ref ID J:92453)

• increased double-negative T cell number
  • the proportion but not absolute number of double negative T cells found in the thymus is increased
  (MGI Ref ID J:92453)

• increased IgG2a level
  • mice infected with Syphacia obvelata pinworm have higher serum levels of IgG2a
  (MGI Ref ID J:92453)

• thymus hypoplasia
  • thymus is visibly smaller with 4 to 5 fold less cells than controls
  (MGI Ref ID J:92453)

• abnormal thymocyte activation
  • double positive thymocytes have greater increases in calcium concentration flux when activated through their TCR complex
  (MGI Ref ID J:92453)
  • single positive thymocytes have dramatically lower fluxes in intracellular calcium concentrations when activated through their TCR complex
  (MGI Ref ID J:92453)

• increased CD4-positive, alpha beta T cell number
  • the proportion but not absolute number of CD4 T cells found in the thymus is increased
  (MGI Ref ID J:92453)

• abnormal T cell activation
  • CD4 T cells in the spleen or lymph nodes have significantly less calcium flux when activated
  (MGI Ref ID J:92453)

• decreased double-positive T cell number
  • the proportion and absolute number of double positive T cells is greatly reduced in the thymus
  (MGI Ref ID J:92453)

• abnormal T cell number
  (MGI Ref ID J:92453)

• abnormal thymus cell ratio
  • the ratio between CD4^-CD8⁺/ CD4⁺CD8^- T cells exceeds 1 compared to 0.3 in controls
  (MGI Ref ID J:92453)

• abnormal thymus morphology
  (MGI Ref ID J:92453)

• thymus cortex hypoplasia
  • thymus lacks a clearly defined cortical region with thymocytes spread out through the medulla in a loosely packed manner
  (MGI Ref ID J:92453)

homeostasis/metabolism phenotype

• increased circulating phosphate level
  • mice are slightly hyperphosphatemic with values of 9.2 mg/dl compared to 8.1 mg/dl in controls
  (MGI Ref ID J:107359)

immune system phenotype

• abnormal T cell activation
  • CD4 T cells in the spleen or lymph nodes have significantly less calcium flux when activated
  (MGI Ref ID J:92453)

• abnormal thymocyte activation
  • single positive thymocytes have dramatically lower fluxes in intracellular calcium concentrations when activated through their TCR complex
  (MGI Ref ID J:92453)
  • double positive thymocytes have greater increases in calcium concentration flux when activated through their TCR complex
  (MGI Ref ID J:92453)

• absent CD8-positive, alpha-beta T cells
  • CD8 T cells are absent in the spleen, lymph nodes, and in circulation
  (MGI Ref ID J:92453)

• abnormal T cell differentiation
  • an increased proportion of CD3⁺ thymocytes co-express alphabeta TCR in the thymus of 6 week old mice
  (MGI Ref ID J:92453)

• abnormal thymus cell ratio
  • the ratio between CD4^-CD8⁺/ CD4⁺CD8^- T cells exceeds 1 compared to 0.3 in controls
  (MGI Ref ID J:92453)

• thymus hypoplasia
  • thymus is visibly smaller with 4 to 5 fold less cells than controls
  (MGI Ref ID J:92453)

• abnormal T cell number
  (MGI Ref ID J:92453)

• thymus cortex hypoplasia
  • thymus lacks a clearly defined cortical region with thymocytes spread out through the medulla in a loosely packed manner
  (MGI Ref ID J:92453)

• abnormal osteoclast physiology
  • tartate-resistant acid phosphatase activity by osteoclasts is markedly reduced
  (MGI Ref ID J:107359)

• abnormal thymus morphology
  (MGI Ref ID J:92453)

• decreased double-positive T cell number
  • the proportion and absolute number of double positive T cells is greatly reduced in the thymus
  (MGI Ref ID J:92453)

• increased interleukin-4 secretion
  • unstimulated thymocytes isolated from mice as young as E18 secrete IL-4 in culture
  (MGI Ref ID J:92453)
  • the amount of IL-4 secreted is 25 to 70 pg/ml over a 24 hour period
  (MGI Ref ID J:92453)

• increased susceptibility to parasitic infection
  • conventionally housed transgenic mice in one facility invariably developed severe intestinal infection with the Syphacia obvelata pinworm
  (MGI Ref ID J:92453)
  • megacolon, rectal prolapse, and cecal perforation were frequent with this infection and mice invariably died between 6-12 weeks of age
  (MGI Ref ID J:92453)

• increased CD8-positive, alpha-beta T cell number
  • the proportion and absolute number of CD8 T cells is greatly increased in the thymus
  (MGI Ref ID J:92453)

• increased double-negative T cell number
  • the proportion but not absolute number of double negative T cells found in the thymus is increased
  (MGI Ref ID J:92453)

• decreased CD4-positive, alpha beta T cell number
  • there is a 5-fold drop in the number of CD4 T cells found in lymph nodes
  (MGI Ref ID J:92453)

• increased CD4-positive, alpha beta T cell number
  • the proportion but not absolute number of CD4 T cells found in the thymus is increased
  (MGI Ref ID J:92453)

• increased IgG2a level
  • mice infected with Syphacia obvelata pinworm have higher serum levels of IgG2a
  (MGI Ref ID J:92453)

limbs/digits/tail phenotype

• increased diameter of femur
  • the medial-lateral cross section is not increased giving the bones a more rounded appearance
  (MGI Ref ID J:107359)
  • the anterior-posterior cross section of femurs is significantly increased
  (MGI Ref ID J:107359)

skeleton phenotype

• abnormal osteoblast physiology
  • osteocalcin level in sera are half that of wild-type mice indicating less osteoblast activity
  (MGI Ref ID J:107359)
  • there is a profound decrease in alkaline phosphatase activity of osteoblasts lining the periosteum, endosteum, and trabeculae
  (MGI Ref ID J:107359)
  • percent of bone surface labeled in vivo with tetracycline is 32.2% versus 49.0% in wild-type controls
  (MGI Ref ID J:107359)

• decreased compact bone thickness
  • there is a significant reduction in the mean cortical thickness and percent cortical area at the midshaft of the radius and the ulna
  (MGI Ref ID J:107359)
  • cortical thinning of the skeleton occurs by 2 months of age with bones looking translucent upon necropsy
  (MGI Ref ID J:107359)

• increased diameter of femur
  • the anterior-posterior cross section of femurs is significantly increased
  (MGI Ref ID J:107359)
  • the medial-lateral cross section is not increased giving the bones a more rounded appearance
  (MGI Ref ID J:107359)

• osteoporosis
  • decreased bone mass but no excess of unmineralized matrix indicates these mice are osteoperotic
  (MGI Ref ID J:107359)

• abnormal osteoclast physiology
  • tartate-resistant acid phosphatase activity by osteoclasts is markedly reduced
  (MGI Ref ID J:107359)

• decreased bone strength
  • both the stiffness and the failure load of femurs is significantly reduced
  (MGI Ref ID J:107359)

• decreased trabecular bone mass
  • trabecular mass in vertebrae and long bones is substantially decreased
  (MGI Ref ID J:107359)

• kyphosis
  • between 3 to 6 months of age, mice develop an exaggerated curvature of the spine
  (MGI Ref ID J:107359)

endocrine/exocrine gland phenotype

• thymus hypoplasia
  • thymus is visibly smaller with 4 to 5 fold less cells than controls
  (MGI Ref ID J:92453)

• abnormal thymocyte activation
  • single positive thymocytes have dramatically lower fluxes in intracellular calcium concentrations when activated through their TCR complex
  (MGI Ref ID J:92453)
  • double positive thymocytes have greater increases in calcium concentration flux when activated through their TCR complex
  (MGI Ref ID J:92453)

• thymus cortex hypoplasia
  • thymus lacks a clearly defined cortical region with thymocytes spread out through the medulla in a loosely packed manner
  (MGI Ref ID J:92453)

• abnormal thymus morphology
  (MGI Ref ID J:92453)

• abnormal thymus cell ratio
  • the ratio between CD4^-CD8⁺/ CD4⁺CD8^- T cells exceeds 1 compared to 0.3 in controls
  (MGI Ref ID J:92453)