B6;129S-Apoc3^tm1Unc/J

Stock number: 002227
Product name: apoc3-
Research areas: Cardiovascular Research

Description

These Apoc3 knock-out mice exhibit reduced levels of fasted plasma triglyceride, total plasma cholesterol, and HDL.

Detailed description

Mice homozygous for the Apoc3^tm1Unc show fasted plasma triglyceride levels that are 70% that present in normal wildtype siblings. Levels of total plasma cholesterol and HDL in homozygous mice are slightly reduced compared to normal wildtype silbings. Homozygotes also show hypotriglyceridemia when given the "fat meal test", and are able to clear chylomicrons faster than normal wildtype silbings. Heterozygous mice show fasted plasma triglyceride levels that are reduced compared to normal wildtype mice, though not as severely as in homozygous mice.

Allele

Symbol: Apoc3^tm1Unc
Name: targeted mutation 1, University of North Carolina
MGI accession ID: MGI:1857128
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Apoc3
Marker name: apolipoprotein C-III
Chromosome: 9
Strain of origin: 129P2/OlaHsd
Molecular note: A genomic fragment containing exon 4 was replaced with a neomycin selection cassette. Northern blot analysis indicated that no stable transcript was produced from this allele in homozygous mice. Expression of the Apoa1 and Apoa4 genes that lie in close proximity to Apoc3 are reduced by half in the intestine of homozygotes but not in the liver.