These Apoc3 knock-out mice exhibit reduced levels of fasted plasma triglyceride, total plasma cholesterol, and HDL.
Dr. Nobuyo Maeda, University of North Carolina at Chapel Hill
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Apoc3 | apolipoprotein C-III |
Mice homozygous for the Apoc3tm1Unc show fasted plasma triglyceride levels that are 70% that present in normal wildtype siblings. Levels of total plasma cholesterol and HDL in homozygous mice are slightly reduced compared to normal wildtype silbings. Homozygotes also show hypotriglyceridemia when given the "fat meal test", and are able to clear chylomicrons faster than normal wildtype silbings. Heterozygous mice show fasted plasma triglyceride levels that are reduced compared to normal wildtype mice, though not as severely as in homozygous mice.
Allele Name | targeted mutation 1, University of North Carolina |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | apoc3- |
Gene Symbol and Name | Apoc3, apolipoprotein C-III |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 9 |
Molecular Note | A genomic fragment containing exon 4 was replaced with a neomycin selection cassette. Northern blot analysis indicated that no stable transcript was produced from this allele in homozygous mice. Expression of the Apoa1 and Apoa4 genes that lie in close proximity to Apoc3 are reduced by half in the intestine of homozygotes but not in the liver. |
Mutations Made By | Dr. Nobuyo Maeda, University of North Carolina at Chapel Hill |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the apoc3- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002227 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Homozygous for Apoc3<tm1Unc>, 1 pair minimum |
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