These transgenic mice express 56 copies of metallothionein 1 (Mt1) from the transgene.
IMR Colony, The Jackson Laboratory
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
These mice overexpress metallothionein1 (Mt1), a stress-response protein found to be significantly increased in the liver and kidney during oxidative stress. These mice express 10- to 20-fold higher basal levels of MT protein in pancreas, liver, and stomach, as well as 2- to 6-fold higher MT protein levels in other organs (kidney, intestine, uterus, testes, spleen, heart, and lung) than control mice.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
The "Tg(Mt-1)Bri 174" transgene was designed with a mouse metallothionein 1 gene (modified with an additional 2 nucleotides located 9 nucleotides upstream of the translational initiation codon) flanked by 10kb of 5' and 7kb of 3' regulatory sequences. The construct was injected into fertilized eggs from (C57BL/6 x SJL)F1 female and male origin. Mutant mice were maintained by crossing heterozygous carriers to B6SJLF1 animals. Subsequently B6SJL heterozygotes were mated to CD-1 (outbred strain) and carriers from this mating were bred to produce homozygotes. The resulting strain (on a mixed C57BL/6, SJL, and CD-1 genetic background - see (Stock No. 002209) was backcrossed at least 5 generations to C57BL/6J mice (Stock No. 000664) after arrival at The Jackson Laboratory to generate this congenic strain (Stock No. 002210).
Expressed Gene | Mt1, metallothionein 1, mouse, laboratory |
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Site of Expression |
Allele Name | transgene insertion 174, Ralph L Brinster |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | MT-TG; MTtrans; TgMTI |
Gene Symbol and Name | Tg(Mt1)174Bri, transgene insertion 174, Ralph L Brinster |
Gene Synonym(s) | |
Promoter | Mt1, metallothionein 1, mouse, laboratory |
Expressed Gene | Mt1, metallothionein 1, mouse, laboratory |
Strain of Origin | (C57BL/6 x SJL)F2 |
Chromosome | UN |
Molecular Note | The transgene contains mouse metallothionein 1 sequence with 10kb of upstream sequence and 7kb of downstream sequence. 56 copies of the transgene integrated in this line. |
Mutations Made By | Dr. Ralph Brinster, University of Pennsylvania |
When maintaining a live colony, homozygous mice can be bred. The expected coat color from breeding is black.
When using the B6.Cg-Tg(Mt1)174Bri/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #002210 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Mt1)174Bri/ |
Frozen Mouse Embryo | B6.Cg-Tg(Mt1)174Bri/J | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Mt1)174Bri/J | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Mt1)174Bri/J | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(Mt1)174Bri/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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