Overview

These transgenic mice express 56 copies of metallothionein 1 (Mt1) from the transgene.

Donating Investigator

IMR Colony, The Jackson Laboratory

Genetic overview

Genetic Background	Generation

Tg(Mt1)174Bri

Allele Type
Transgenic (Inserted expressed sequence)

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Research Applications

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

These mice overexpress metallothionein1 (Mt1), a stress-response protein found to be significantly increased in the liver and kidney during oxidative stress. These mice express 10- to 20-fold higher basal levels of MT protein in pancreas, liver, and stomach, as well as 2- to 6-fold higher MT protein levels in other organs (kidney, intestine, uterus, testes, spleen, heart, and lung) than control mice.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development

The "Tg(Mt-1)Bri 174" transgene was designed with a mouse metallothionein 1 gene (modified with an additional 2 nucleotides located 9 nucleotides upstream of the translational initiation codon) flanked by 10kb of 5' and 7kb of 3' regulatory sequences. The construct was injected into fertilized eggs from (C57BL/6 x SJL)F1 female and male origin. Mutant mice were maintained by crossing heterozygous carriers to B6SJLF1 animals. Subsequently B6SJL heterozygotes were mated to CD-1 (outbred strain) and carriers from this mating were bred to produce homozygotes. The resulting strain (on a mixed C57BL/6, SJL, and CD-1 genetic background - see (Stock No. 002209) was backcrossed at least 5 generations to C57BL/6J mice (Stock No. 000664) after arrival at The Jackson Laboratory to generate this congenic strain (Stock No. 002210).

Expression Data

Expressed Gene	Mt1, metallothionein 1, mouse, laboratory
Site of Expression

Control Suggestions

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Palmiter RD; Sandgren EP; Koeller DM; Brinster RL. 1993. Distal regulatory elements from the mouse metallothionein locus stimulate gene expression in transgenic mice. Mol Cell Biol 13(9):5266-75PubMed: 8355681 MGI: J:92452

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Genetics

Tg(Mt1)174Bri

Allele Symbol: Tg(Mt1)174Bri

Allele Name	transgene insertion 174, Ralph L Brinster
Allele Type	Transgenic (Inserted expressed sequence)
Allele Synonym(s)	MT-TG; MTtrans; TgMTI
Gene Symbol and Name	Tg(Mt1)174Bri, transgene insertion 174, Ralph L Brinster
Gene Synonym(s)
Promoter	Mt1, metallothionein 1, mouse, laboratory
Expressed Gene	Mt1, metallothionein 1, mouse, laboratory
Strain of Origin	(C57BL/6 x SJL)F2
Chromosome	UN
Molecular Note	The transgene contains mouse metallothionein 1 sequence with 10kb of upstream sequence and 7kb of downstream sequence. 56 copies of the transgene integrated in this line.
Mutations Made By	Dr. Ralph Brinster, University of Pennsylvania

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Tg(Mt1)174Bri/Tg(Mt1)174Bri
involves: C57BL/6 * SJL

cardiovascular system phenotype

abnormal response to cardiac infarction
- myocardial oxidative damage is reduced by almost two-thirds as measured by levels of lipid peroxidation found in the heart after ischemia/reperfusion
- the mean percentage of apoptotic cells present in the myocardium after ischemia/reperfusion is 14.4% compared to 26.3%

decreased myocardial infarction size
- area of tissue with infarctions visible is only about half that of non-transgenic controls
- only small scattered infarct zones are present after ischemia/reperfusion-induced myocardial infarction

digestive/alimentary phenotype

abnormal intestinal mineral absorption
- 2 hours after oral dosing with zinc, mice have about a 2-fold less zinc concentration in the blood than in control mice

homeostasis/metabolism phenotype

decreased myocardial infarction size
- area of tissue with infarctions visible is only about half that of non-transgenic controls
- only small scattered infarct zones are present after ischemia/reperfusion-induced myocardial infarction

abnormal response to cardiac infarction
- the mean percentage of apoptotic cells present in the myocardium after ischemia/reperfusion is 14.4% compared to 26.3%
- myocardial oxidative damage is reduced by almost two-thirds as measured by levels of lipid peroxidation found in the heart after ischemia/reperfusion

Genotype: Tg(Mt1)174Bri/0
involves: C57BL/6 * SJL

homeostasis/metabolism phenotype

abnormal mineral level
- mice have 50% more zinc in the liver and 3-fold more zinc in the pancreas than is found in non-transgenic littermate controls

The following phenotype relates to a compound genotype created using this strain

Genotype: Tg(Mt1)174Bri/0
B6.Cg-Tg(Mt1)174Bri/J

homeostasis/metabolism phenotype

decreased sensitivity to xenobiotic induced morbidity/mortality
- treated-treated mice exhibit increased mean survival time compared with similarly treated wild-type mice

mortality/aging

decreased sensitivity to xenobiotic induced morbidity/mortality
- treated-treated mice exhibit increased mean survival time compared with similarly treated wild-type mice

References

Palmiter RD; Sandgren EP; Koeller DM; Brinster RL. 1993. Distal regulatory elements from the mouse metallothionein locus stimulate gene expression in transgenic mice. Mol Cell Biol 13(9):5266-75PubMed: 8355681 MGI: J:92452

Additional References

Kang YJ; Li Y; Sun X; Sun X. 2003. Antiapoptotic effect and inhibition of ischemia/reperfusion-induced myocardial injury in metallothionein-overexpressing transgenic mice. Am J Pathol 163(4):1579-86PubMed: 14507664 MGI: J:85798
Waelput W; Broekaert D; Vandekerckhove J; Brouckaert P; Tavernier J; Libert C. 2001. A mediator role for metallothionein in tumor necrosis factor-induced lethal shock. J Exp Med 194(11):1617-24PubMed: 11733576 MGI: J:73099

Additional - Tg(Mt1)174Bri related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Tg(Mt1)174Bri
- Standard PCR:Tg(Mt1)174Bri
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, homozygous mice can be bred. The expected coat color from breeding is black.
- Additional Breeding and Husbandry Support
Appearance
- black
  Related Genotype: a/a
Citation
When using the B6.Cg-Tg(Mt1)174Bri/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #002210 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Hemizygous or Non carrier for Tg(Mt1)174Bri/

Related Products and Services

Frozen Mouse Embryo	B6.Cg-Tg(Mt1)174Bri/J	$2595.00
Frozen Mouse Embryo	B6.Cg-Tg(Mt1)174Bri/J	$2595.00
Frozen Mouse Embryo	B6.Cg-Tg(Mt1)174Bri/J	$3373.50
Frozen Mouse Embryo	B6.Cg-Tg(Mt1)174Bri/J	$3373.50

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Tg(Mt1)174Bri

Allele Symbol: Tg(Mt1)174Bri

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Tg(Mt1)174Bri/Tg(Mt1)174Briinvolves: C57BL/6 * SJL

cardiovascular system phenotype

digestive/alimentary phenotype

homeostasis/metabolism phenotype

Genotype: Tg(Mt1)174Bri/0involves: C57BL/6 * SJL

homeostasis/metabolism phenotype

Genotype: Tg(Mt1)174Bri/0B6.Cg-Tg(Mt1)174Bri/J

homeostasis/metabolism phenotype

mortality/aging

References

Additional References

Additional - Tg(Mt1)174Bri related

Genotyping Protocols

Breeding Considerations

Appearance

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

Genotype: Tg(Mt1)174Bri/Tg(Mt1)174Bri
involves: C57BL/6 * SJL

Genotype: Tg(Mt1)174Bri/0
involves: C57BL/6 * SJL

Genotype: Tg(Mt1)174Bri/0
B6.Cg-Tg(Mt1)174Bri/J