Mice homozygous for this mutation die at mid-gestation. The embryos are stunted and show a delayed development. DNA from these mice shows a reduction in the level of m5C to about 1/3 that seen in cells from normal wildtype siblings.
IMR Colony, The Jackson Laboratory
Mice homozygous for this mutation die at mid gestation. The embryos are stunted and show a delayed development. DNA from these mice shows a reduction in the level of m5C to about 1/3 that seen in cells from normal wildtype siblings.
A targeting vector consisting of a neomycin cassette replacement of 900 bp, including the translation start site, was electroporated into J1 ES cells. ES cells were injected into C57BL/6J blastocysts and chimeric male offspring were mated to C57BL/6 females.
|Allele Name||targeted mutation 1, Rudolf Jaenisch|
|Allele Type||Targeted (Hypomorph)|
|Allele Synonym(s)||Dmntn; DnmtN; Dnmt1N; MTase(-); Mtasen|
|Gene Symbol and Name||Dnmt1, DNA methyltransferase (cytosine-5) 1|
|Strain of Origin||129S4/SvJae|
|Molecular Note||Replacement of 900 bp, including the translation start site, with a neomycin cassette. This is a hypomorphic allele; enzyme activity levels have dropped to approximately 30% of the normal value.|
|Mutations Made By|| |
Rudolf Jaenisch, Whitehead Institute, Massachusetts Institute of Technology
While maintaining a live colony, these mice are bred as heterozygotes. Mice homozygous for the mutation are not viable.
When using the B6.129S4-Dnmt1tm1Jae/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #002198 in your Materials and Methods section.