These lacZ transgenic mice carry no obvious phenotype. The protein product is localized to the cell nucleus as a result of the presence of the SV40 large T-antigen nuclear localization signal peptide. Β-Galactosidase activity is detected in hepatocytes of the liver.
Dr. Ralph Brinster, University of Pennsylvania
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter) |
These lacZ transgenic mice carry no obvious phenotype. The protein product is localized to the cell nucleus as a result of the presence of the SV40 large T-antigen nuclear localization signal peptide. Β-Galactosidase activity is detected in hepatocytes of the liver. Expression can be induced up to 10-fold by administration of heavy metal ions.
The mouse Mt1 (metallothionein) promoter and flanking DNA (-588 to +68) were fused to the bacterial lacZ gene with a nuclear localization sequence. The construct was inserted into the EcoRI site of the vector that carries 10kb of sequence flanking Mt2 and 7kb flanking the Mt1 gene. These flanking sequences contain ribonuclease-hypersensitive sites that appear to be important for enhanced expression.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression | lacZ is expressed in hepatocytes. Protein product is localized to cell nucleus by SV40 large T-antigen nuclear localization signal peptide. |
Allele Name | transgene insertion 204, Ralph L Brinster |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | |
Gene Symbol and Name | Tg(MTn-lacZ)204Bri, transgene insertion 204, Ralph L Brinster |
Gene Synonym(s) | |
Promoter | Mt1, metallothionein 1, mouse, laboratory |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | lacZ is expressed in hepatocytes. Protein product is localized to cell nucleus by SV40 large T-antigen nuclear localization signal peptide. |
Strain of Origin | C57BL/6J |
Chromosome | UN |
General Note | Transgenic mice on a C57BL/6J background display no obvious phenotype. The protein product is localized to the cell nucleus. Expression is present in the liver of transgenic mice as a result of the presence of the SV40 large T-antigen nuclear localization signal peptide. Hepatocytes stain blue with X-gal staining. Expression can be induced up to 10-fold by administration of heavy metal ions. |
Molecular Note | The mouse Mt1 (metallothionein 1) promoter and flanking DNA (-588 to +68) were fused to the bacterial lacZ gene with a nuclear localization sequence. The construct was inserted into the EcoRI site of a vector that carries 10kb of sequence flanking Mt2 and 7kb flanking the Mt1 gene. These flanking sequences contain ribonuclease-hypersensitive sites thought to be important for enhanced expression. |
This colony is maintained by homozygous sibling matings. Expected coat color from breeding:Black
When using the C57BL/6J-Tg(MTn-lacZ)204Bri/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #002193 in your Materials and Methods section.
Facility Barrier Level Descriptions
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Homozygous for Tg(MTn-lacZ)204Bri |
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