C57BL/6J-Tg(MTn-lacZ)204Bri/J

Stock number: 002193
Research areas: Research Tools

Description

These lacZ transgenic mice carry no obvious phenotype. The protein product is localized to the cell nucleus as a result of the presence of the SV40 large T-antigen nuclear localization signal peptide. Β-Galactosidase activity is detected in hepatocytes of the liver.

Detailed description

These lacZ transgenic mice carry no obvious phenotype. The protein product is localized to the cell nucleus as a result of the presence of the SV40 large T-antigen nuclear localization signal peptide. Β-Galactosidase activity is detected in hepatocytes of the liver. Expression can be induced up to 10-fold by administration of heavy metal ions.

Development

The mouse Mt1 (metallothionein) promoter and flanking DNA (-588 to +68) were fused to the bacterial lacZ gene with a nuclear localization sequence. The construct was inserted into the EcoRI site of the vector that carries 10kb of sequence flanking Mt2 and 7kb flanking the Mt1 gene. These flanking sequences contain ribonuclease-hypersensitive sites that appear to be important for enhanced expression.

Allele

Symbol: Tg(MTn-lacZ)204Bri
Name: transgene insertion 204, Ralph L Brinster
MGI accession ID: MGI:3052912
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(MTn-lacZ)204Bri
Marker name: transgene insertion 204, Ralph L Brinster
Chromosome: UN
Strain of origin: C57BL/6J
Expressed genes: lacZ,beta-galactosidase,E. coli
Site of expression: lacZ is expressed in hepatocytes. Protein product is localized to cell nucleus by SV40 large T-antigen nuclear localization signal peptide.
Molecular note: The mouse Mt1 (metallothionein 1) promoter and flanking DNA (-588 to +68) were fused to the bacterial lacZ gene with a nuclear localization sequence. The construct was inserted into the EcoRI site of a vector that carries 10kb of sequence flanking Mt2 and 7kb flanking the Mt1 gene. These flanking sequences contain ribonuclease-hypersensitive sites thought to be important for enhanced expression.
General note: Transgenic mice on a C57BL/6J background display no obvious phenotype. The protein product is localized to the cell nucleus. Expression is present in the liver of transgenic mice as a result of the presence of the SV40 large T-antigen nuclear localization signal peptide. Hepatocytes stain blue with X-gal staining. Expression can be induced up to 10-fold by administration of heavy metal ions.