Mice hemizygous or homozygous for the ROSA26 retroviral insertion display no distinguishing phenotype. lacZ is expressed in all tissues of the developing embryo and in most tissues of the adult mouse.
Dr. William F. Dove, University of Wisconsin- Madison
Genetic Background | Generation |
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000664 C57BL/6J |
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Allele Type | Gene Symbol | Gene Name |
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Gene trapped (Not Applicable) | Gt(ROSA)26Sor | gene trap ROSA 26, Philippe Soriano |
Mice heterozygous or homozygous for the ROSA26 retroviral insertion display no distinguishing phenotype. lacZ is expressed in all tissues of the developing embryo and in most tissues of the adult mouse.
If breeding heterozygous mice together, recovery of homozygous offspring is significantly reduced. For unknown reasons, homozygous mutant mice are prone to convulsions. If breeding or creating homozygous mice, they should be handled carefully and quietly to avoid poor breeding, loss of litters, or premature death.
This strain was developed in the laboratory of Dr. Philippe Soriano at the Fred Hutchinson Cancer Center, Seattle, WA. This mutant was made by a retroviral insertion of the beta-galactosidase gene (and accompanying splice acceptor and neo sequences) into ES cells. The construct was inserted in reverse orientation in the retroviral vector pGen-.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression | All tissues of the developing embryo and in most tissues of the adult mouse. |
Allele Name | gene trap ROSA 26, Philippe Soriano |
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Allele Type | Gene trapped (Not Applicable) |
Allele Synonym(s) | gtrosa26; R26tg; R26R3; ROSA26; Rosa26lacZ; ROSA26-lacZ; TgR(ROSA26)26Sor |
Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | All tissues of the developing embryo and in most tissues of the adult mouse. |
Strain of Origin | 129S7/SvEvBrd-Hprt+ |
Chromosome | 6 |
General Note | ES cell line = AB1 or AB2.1. No overt phenotypes in heterozygous or homozygous animals. The trapped gene appears to be ubiquitously expressed during embryonic development and in all hematopoietic cells. |
Molecular Note | Random insertion of retroviral gene trap vector ROSA, Reverse Orientation with Splice Acceptor, which contains the beta-galactosidase gene and accompanying splice acceptor and neo sequences. The construct was inserted in reverse orientation in the retroviral vector pGen-. The transgene is controlled by an unknown endogenous promoter. lacZ is expressed in all tissues of the developing embryo and in most tissues of the adult transgenic mouse. |
Mutations Made By | Dr. Philippe Soriano, Mount Sinai School of Medicine |
The live colony at The Jackson Laboratory Repository is maintained by breeding heterozygous mice with wildtype mice from the colony or with C57BL/6J inbred mice (Stock No. 000664). The expected coat color from breeding is black.
Heterozygous mice and wildtype mice from the colony may be ordered from The Jackson Laboratory Repository. We also offer heterozygous breeding pairs for distribution (although we do not maintain our colony in this manner). If breeding heterozygous mice together, recovery of homozygous offspring is significantly reduced. For unknown reasons, homozygous mutant mice are prone to convulsions. If breeding or creating homozygous mice, they should be handled carefully and quietly to avoid poor breeding, loss of litters, or premature death.
When using the ROSA26-lacZ mouse strain in a publication, please cite the originating article(s) and include JAX stock #002192 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Gt(ROSA)26Sor |
Frozen Mouse Embryo | B6.129S7-Gt(ROSA)26Sor/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S7-Gt(ROSA)26Sor/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S7-Gt(ROSA)26Sor/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129S7-Gt(ROSA)26Sor/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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