B6.129S7-Gt(ROSA)26Sor/J

Stock number: 002192
Product name: ROSA26-lacZ
Research areas: Research Tools

Description

Mice hemizygous or homozygous for the ROSA26 retroviral insertion display no distinguishing phenotype. lacZ is expressed in all tissues of the developing embryo and in most tissues of the adult mouse.

Detailed description

Mice heterozygous or homozygous for the ROSA26 retroviral insertion display no distinguishing phenotype. lacZ is expressed in all tissues of the developing embryo and in most tissues of the adult mouse.

If breeding heterozygous mice together, recovery of homozygous offspring is significantly reduced. For unknown reasons, homozygous mutant mice are prone to convulsions. If breeding or creating homozygous mice, they should be handled carefully and quietly to avoid poor breeding, loss of litters, or premature death.

Development

This strain was developed in the laboratory of Dr. Philippe Soriano at the Fred Hutchinson Cancer Center, Seattle, WA. This mutant was made by a retroviral insertion of the beta-galactosidase gene (and accompanying splice acceptor and neo sequences) into ES cells. The construct was inserted in reverse orientation in the retroviral vector pGen-.

Allele

Symbol: Gt(ROSA)26Sor
Name: gene trap ROSA 26, Philippe Soriano
MGI accession ID: MGI:1890203
Generation method: Gene trapped
Attributes: Not Applicable
Marker symbol: Gt(ROSA)26Sor
Marker name: gene trap ROSA 26, Philippe Soriano
Chromosome: 6
Strain of origin: 129S7/SvEvBrd-Hprt⁺
Expressed genes: lacZ,beta-galactosidase,E. coli
Site of expression: All tissues of the developing embryo and in most tissues of the adult mouse.
Molecular note: Random insertion of retroviral gene trap vector ROSA, Reverse Orientation with Splice Acceptor, which contains the beta-galactosidase gene and accompanying splice acceptor and neo sequences. The construct was inserted in reverse orientation in the retroviral vector pGen-. The transgene is controlled by an unknown endogenous promoter. lacZ is expressed in all tissues of the developing embryo and in most tissues of the adult transgenic mouse.
General note: ES cell line = AB1 or AB2.1. No overt phenotypes in heterozygous or homozygous animals. The trapped gene appears to be ubiquitously expressed during embryonic development and in all hematopoietic cells.