The transgenic construct consists of a 990 bp fragment corresponding to the full length antisense murine Il3 cDNA under the control of the B19 parvovirus promoter and a 3’ T cell dominant control region from the 3’ end of the human CD2 gene. The construct was injected into fertilized eggs from FVB/N female mice mated to CB6F1 males in the laboratory of Dr. Cynthia Dunbar at the NIH. Founder line 241 exhibits the highest penetrance of the two phenotypes. This transgenic was crossed to a strain designated KIV to generate heterozygosity at the Hprt locus. (Unfortunately, we do not have information regarding the origins or characteristics of strain KIV.) The strain was then backcrossed to FVB/N for three generations. The donating investigator reports that these transgenic mice manifest a phenotype identical to the strain reported in Cockayne DA et al. Blood 84:2699 (1994). In 1993, The Jackson Laboratory imported this line and mated transgenic males to FVB/NMob females for an additional backcross. The line was cryopreserved in 1994.
|Expressed Gene||Il3, antisense RNA,|
|Site of Expression|
|Allele Name||transgene insertion 241, Debra A Cockayne|
|Allele Type||Transgenic (Inserted expressed sequence)|
|Gene Symbol and Name||Tg(B19-RNAi:Il3)241Ckn, transgene insertion 241, Debra A Cockayne|
|Gene Synonym(s)||AS-IL-3; Tg(B19-Il3)241Ckn|
|Promoter||B19, B19 parvovirus, virus|
|Expressed Gene||Il3, antisense RNA,|
|Strain of Origin||FVB/N X (BALB/c x C57BL/6)F1|
|General Note||Of 5 transgenic founder lines, two exhibited no abnormal phenotype and were not further characterized. One of the other founder mice died of neurologic disease at 3 months, without having bred. Founders 241 and 244 transmitted the transgene and both the lymphoproliferative and neurologic phenotypes to their progeny. The pathology of the neurologic and of the lymphoproliferative disorders differs between the two lines only in penetrance and in age of onset (3-6 months for line 241 compared to 6-12 months for line 244).|
|Molecular Note||This transgene consists of the full length, 990-bp mouse interleukin 3 cDNA, in reverse (antisense) orientation, under control of the B19 parvovirus promoter and a T-cell dominant control region from the 3' end of the human CD2 gene. A human beta-globin gene segment including the 3' end of exon 2, all of intron 2 and exon 3, the polyadenylation signal and some 3' flanking DNA is present just downstream of the Il3 sequence. Interleukin 3 antisense RNA is detected by RT-PCR analysis in mature CD3+ T lymphocytes of transgenic animals both with lymphoproliferation and with no disease phenotype, in transgenic B220+IgM- pre-B cells, and in brains of both asymptomatic and neurologically affected transgenic mice. Il3 antisense transcripts are present in cultured splenocytes from asymptomatic transgenic mice following in vitro concanavalin A (ConA) stimulation, and ELISA of supernatants from these cultures demonstrates a 5-fold reduction in interleukin 3 secretion compared to similar cultures of control splenocytes.|
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided,
their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of
each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders
are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in
order to provide the minimum number of animals, animals will ship within 25 weeks.
The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
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