Homozygous mutant mice show an increased neutrophil count, and a decreased inflammatory response to peritonitis. Responses to delayed-type hypersensitivity and rejection of transplanted tissue are impaired. This strain serves as a model for the moderate form of human CD18 deficiency.
Dr. Arthur Beaudet, Baylor College of Medicine
Genetic Background | Generation |
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000664 C57BL/6J |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Hypomorph) | Itgb2 | integrin beta 2 |
Mice homozygous for the Itgb2tm1Bay mutation are viable and fertile. Homozygous mutant mice show an increased neutrophil count, and a decreased inflammatory response to peritonitis. Responses to delayed-type hypersensitivity and rejection of transplanted tissue are impaired. PLEASE NOTE: The Itgb2tm1Bay allele is not a null mutation; it is a hypomorphic mutation which results in the expression of very low levels of Itgb2 protein. This strain serves as a model for the moderate form of human CD18 deficiency.
The Itgb2tm1Bay mutant strain was developed in the laboratory of Dr. Arthur Beaudet at Baylor College of Medicine. The 129-derived AB1 ES cell line was used. The C57BL/6J strain was produced by backcrossing the Itgb2tm1Bay mutation 10 times to C57BL/6J inbred mice.
Allele Name | targeted mutation 1, Baylor College of Medicine |
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Allele Type | Targeted (Hypomorph) |
Allele Synonym(s) | CD18-; CD18hypo; CD18low; Itgb2hypo |
Gene Symbol and Name | Itgb2, integrin beta 2 |
Gene Synonym(s) | |
Strain of Origin | 129S7/SvEvBrd-Hprt+ |
Chromosome | 10 |
Molecular Note | An insertion mutation that duplicates exons 2 and 3 with interruption of one copy of exon 3 with a neomycin cassette. This is not a null mutation but rather a hypomorph expressing very low levels of ITGB2 protein. |
Mutations Made By | Dr. Arthur Beaudet, Baylor College of Medicine |
The Itgb2tm1Bay strain is maintained by homozygous sibling matings. Homozygous mice may be ordered. Expected coat color from breeding:Black
When using the CD18- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002128 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Homozygous for Itgb2<tm1Bay>, 1 pair minimum |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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