B6.129S7-Itgb2^tm1Bay/J

Stock number: 002128
Product name: CD18^- (hypomorph)
Research areas: Cell Biology Research, Dermatology Research, Developmental Biology Research, Immunology, Inflammation and Autoimmunity Research, Mouse/Human Gene Homologs

Description

Homozygous mutant mice show an increased neutrophil count, and a decreased inflammatory response to peritonitis. Responses to delayed-type hypersensitivity and rejection of transplanted tissue are impaired.
PLEASE NOTE: The Itgb2^tm1Bay allele is not a null mutation; it is a hypomorphic mutation which results in the expression of very low levels of Itgb2 protein. This strain serves as a model for the moderate form of human CD18 deficiency.

Detailed description

Mice homozygous for the Itgb2^tm1Bay mutation are viable and fertile. Homozygous mutant mice show an increased neutrophil count, and a decreased inflammatory response to peritonitis. Responses to delayed-type hypersensitivity and rejection of transplanted tissue are impaired. PLEASE NOTE: The Itgb2^tm1Bay allele is not a null mutation; it is a hypomorphic mutation which results in the expression of very low levels of Itgb2 protein. This strain serves as a model for the moderate form of human CD18 deficiency.

Development

The Itgb2^tm1Bay mutant strain was developed in the laboratory of Dr. Arthur Beaudet at Baylor College of Medicine. The 129-derived AB1 ES cell line was used. The C57BL/6J strain was produced by backcrossing the Itgb2^tm1Bay mutation 10 times to C57BL/6J inbred mice.

Allele

Symbol: Itgb2^tm1Bay
Name: targeted mutation 1, Baylor College of Medicine
MGI accession ID: MGI:1857208
Generation method: Targeted
Attributes: Hypomorph
Synonyms: CD18^-; CD18^hypo; CD18^low; Itgb2^hypo
Marker symbol: Itgb2
Marker name: integrin beta 2
Marker synonyms: 2E6; Cd18; LAD; LCAMB; LFA-1; MAC-1; Mac-1 beta; MF17; MFI7
Chromosome: 10
Strain of origin: 129S7/SvEvBrd-Hprt1⁺
Molecular note: An insertion mutation that duplicates exons 2 and 3 with interruption of one copy of exon 3 with a neomycin cassette. This is not a null mutation but rather a hypomorph expressing very low levels of ITGB2 protein.