Mice harboring this T-cell receptor delta chain (Tcrd) knock-out are deficient in receptor expression in all adult lymphoid and epithelial organs, and these mice may be useful for studying immunology and gamma delta T-cell function.
IMR Colony, The Jackson Laboratory
Mice homozygous for the Tcrdtm1Mom targeted mutation are viable and fertile. Gamma delta T-cell receptor expression is deficient in all adult lymphoid and epithelial organs. There is normal development of the alpha beta T-cell lineage. Patterns of CD4+CD8- and CD4-CD8+ alpha beta T-cells are apparently normal. Mice do not develop inflammatory bowel disease. Diet-induced obesity in homozygous mice results in a reduction in insulin resistance and liver and skeletal muscle inflammation as compared to C57BL/6J controls fed high fat diet (42% fat). This strain may be useful for studying gamma delta T cells and their role in the proinflammatory response.
The Tcrdtm1Mom targeted mutant strain was developed by Dr. Peter Mombaerts in the laboratory of Dr. Susumu Tonegawa at the Center for Cancer Research, Massachusetts Institute of Technology. A replacement targeting vector containing Pgk-neo was used which resulted in a 4 kb deletion encompassing most of the delta chain constant region coding sequences. The C57BL/6J strain was generated by backcrossing mice carrying the Tcrdtm1Mom mutation 12 times to C57BL/6J inbred mice.
|Allele Name||targeted mutation 1, Peter Mombaerts|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||C-delta-; delta-; delta0; gammadelta TCR-; TCRdelta-; TCRdelta KO|
|Gene Symbol and Name||Tcrd, T cell receptor delta chain|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||A PGK-neo cassette was used to disrupt the constant gene segment (designated C-delta). Flow cytometry showed that cells isolated from the lymphoid or epithelial organs of homozygous mice did not react with an anti-pan Tcrd mAb. Immunoprecipitation showed an absence of expression on the surface of thymocytes isolated from homozygous mutant embryos.|
|Mutations Made By|| |
Peter Mombaerts, Max Planck Research Unit for Neurogenetics
This Tcrdtm1Mom strain is maintained by mating homozygous siblings. This strain should be housed under conditions similar to Prkdcscid/Prkdcscid mice or any other immunodeficient strain. Only homozygous mice may be ordered. Expected coat color from breeding:Black
When using the B6-TCRdelta- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002120 in your Materials and Methods section.