Mice homozygous mice for the Tcratm1Mom targeted mutation are deficient in the alpha beta T-cell receptor. The thymus is devoid of CD4+CD8- and CD4-CD8+ cells. Mice may develop inflammatory bowel disease beginning at ~4-6 months of age.
Peter Mombaerts, Max Planck Research Unit for Neurogenetics
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Tcra | T cell receptor alpha chain |
Mice homozygous mice for the Tcratm1Mom targeted mutation are viable and fertile. They are deficient in the alpha beta T-cell receptor. The thymus is devoid of CD4+CD8- and CD4-CD8+ cells. Normal numbers of CD4+CD8+ cells are retained without the IL2 receptor. There are normal numbers of CD4-CD8- cells. ~1% of the thymocytes express the gamma delta TCR. Mice may develop inflammatory bowel disease beginning at ~4-6 months of age.
The Tcratm1Mom mutant strain was developed by Dr. Peter Mombaerts in the laboratory of Dr. Susumu Tonegawa at the Center for Cancer Research, Massachusetts Institute of Technology. A targeting vector with the Pgk-neo gene disrupting exon 1 of Tcra was used. The 129-derived D3 ES cell line was used.
Allele Name | targeted mutation 1, Peter Mombaerts |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | alpha-; C-alpha-; TCR alpha-; TCR alpha0; TCR Calpha-; TCRmini; TCRalpha-; TCRalpha KO |
Gene Symbol and Name | Tcra, T cell receptor alpha chain |
Gene Synonym(s) | |
Strain of Origin | 129S2/SvPas |
Chromosome | 14 |
Molecular Note | A PGK-neo cassette was used to disrupt exon 1, which encodes the majority of the extracellular domain. The targeting event was confirmed by Southern analysis. |
Mutations Made By | Peter Mombaerts, Max Planck Research Unit for Neurogenetics |
When held in a live colony, this Tcratm1Mom strain is maintained by mating homozygous siblings. This strain should be housed under conditions similar to Prkdcscid/Prkdcscid mice or any other immunodeficient strain. It has been cryopreserved and is currently only available from the frozen embryo repository.
When using the B6;129S2-Tcratm1Mom/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #002115 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Homozygous for Tcra<tm1Mom>, 1 pair minimum |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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