B6.Cg-Gusb^mps Tg(Gus^sx)1Wat/J

Stock number: 002086
Product name: TgGUS-SX
Research areas: Developmental Biology Research, Metabolism Research, Mouse/Human Gene Homologs, Neurobiology Research, Sensorineural Research

Detailed description

Androgen induction of Gus is faster but lower in M. saxicola than in Mus musculus. However, growth hormone induces Gus upregulation in Mus musculus but has no affect on Gus induction in Mus saxicola. When transferred to a Mus musculus background in the absence of endogenous Gus the Mus saxicola derived Gus (Gus^sx) also showed no growth hormone enhancement of induction supporting the idea that an evolutionary change in regulatory sequence underlies this variation in response. These mice do not exhibit the phenotypic characteristics of Gus^mps homozygotes indicating rescue by Gus^sx. (Niermann and Watson, 1999; Paigen 1989.)

Development

Mus saxicola and Mus musculus are not interfertile. In order to study the expression of Mus saxicola derived Gus in the context of the Mus musculus background, a cosmid clone from Mus saxicola genomic DNA, that included the full Gus gene, approximately 3 kb of 5' sequence, and 17 kb of 3' sequence, was transfected into (B6 x SJL)F2 oocytes then bred to C57BL/6J. The resulting line was estimated to have 4 +/-1 copies of the transgene. This transgenic line was crossed with C57BL/6J-Gus^mps mice to develop the B6.Cg-Gus^mps Tg(Gus^sx)1Wat/J strain which is homozygous for both the transgene and the Gus^mps mutation.

Allele

Symbol: Gusb^mps
Name: beta glucuronidase, mucopolysaccharidosis VII
MGI accession ID: MGI:1856041
Generation method: Spontaneous
Marker symbol: Gusb
Marker name: glucuronidase, beta
Chromosome: 5
Strain of origin: B6.C-H2-K^bm1/By
Molecular note: A 1-bp deletion creates a frameshift mutation within exon 10, which introduces a premature stop codon at codon 497.