Androgen induction of Gus is faster but lower in M. saxicola than in Mus musculus. However, growth hormone induces Gus upregulation in Mus musculus but has no affect on Gus induction in Mus saxicola. When transferred to a Mus musculus background in the absence of endogenous Gus the Mus saxicola derived Gus (Gussx) also showed no growth hormone enhancement of induction supporting the idea that an evolutionary change in regulatory sequence underlies this variation in response. These mice do not exhibit the phenotypic characteristics of Gusmps homozygotes indicating rescue by Gussx. (Niermann and Watson, 1999; Paigen 1989.)
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Spontaneous | Gusb | glucuronidase, beta |
Androgen induction of Gus is faster but lower in M. saxicola than in Mus musculus. However, growth hormone induces Gus upregulation in Mus musculus but has no affect on Gus induction in Mus saxicola. When transferred to a Mus musculus background in the absence of endogenous Gus the Mus saxicola derived Gus (Gussx) also showed no growth hormone enhancement of induction supporting the idea that an evolutionary change in regulatory sequence underlies this variation in response. These mice do not exhibit the phenotypic characteristics of Gusmps homozygotes indicating rescue by Gussx. (Niermann and Watson, 1999; Paigen 1989.)
Mus saxicola and Mus musculus are not interfertile. In order to study the expression of Mus saxicola derived Gus in the context of the Mus musculus background, a cosmid clone from Mus saxicola genomic DNA, that included the full Gus gene, approximately 3 kb of 5' sequence, and 17 kb of 3' sequence, was transfected into (B6 x SJL)F2 oocytes then bred to C57BL/6J. The resulting line was estimated to have 4 +/-1 copies of the transgene. This transgenic line was crossed with C57BL/6J-Gusmps mice to develop the B6.Cg-Gusmps Tg(Gussx)1Wat/J strain which is homozygous for both the transgene and the Gusmps mutation.
Allele Name | beta glucuronidase, mucopolysaccharidosis VII |
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Allele Type | Spontaneous |
Allele Synonym(s) | asd; gusmps; Gus-b; MPS VII |
Gene Symbol and Name | Gusb, glucuronidase, beta |
Gene Synonym(s) | |
Strain of Origin | B6.C-H2-Kbm1/By |
Chromosome | 5 |
Molecular Note | A 1-bp deletion creates a frameshift mutation within exon 10, which introduces a premature stop codon at codon 497. |
Mutations Made By | Brian Soper, The Jackson Laboratory |
When using the TgGUS-SX mouse strain in a publication, please cite the originating article(s) and include JAX stock #002086 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Homozygous for Gusb<mps>, Homozygous for Tg(Gus<sx>)1Wat, 1 pair minimum |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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