Mice homozygous for the Ldlrtm1Her knock-out mutation have elevated serum cholesterol levels and may be useful in studies of atherosclerosis.
Robert E Hammer, UT Southwestern Medical Center at Dallas
Mice homozygous for the Ldlrtm1Her knock-out mutation have an elevated serum cholesterol level of 200-400 mg/dl and they have very high levels (>2,000 mg/dl) when fed a high fat diet. Normal serum cholesterol in the mouse is 80-100 mg/dl.
The Ldlrtm1Her mutant strain was developed in the laboratory of Dr. Robert Hammer and Dr. Joachim Herz at the Howard Hughes Medical Institute Research Laboratories, University of Texas Southwestern Medical Center at Dallas. The 129-derived AB1 ES cell line was used.
|Allele Name||targeted mutation 1, Joachim Herz|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||LDLR-; LDLR KO; LDLr0; LDLrKO; LDLr-KO; Ldlrtm1Her|
|Gene Symbol and Name||Ldlr, low density lipoprotein receptor|
|Site of Expression||Immunoblot analysis of liver membranes detected a truncated protein in homozygous mutant animals.|
|Strain of Origin||129S7/SvEvBrd-Hprt+|
|General Note||When used in bone marrow transplant into Ldlrtm1Her homozygous mice, Abca1tm1Jdm Abcg1tm1Dgen homozygous cells accelerate the development of atherosclerosis. (J:130777) |
|Molecular Note||Insertion of a neomycin resistance cassette into exon 4. The authors predict that the targeted allele would encode a truncated non-functional protein that will not bind LDL, and that lacks a membrane spanning segment. Immunoblot analysis of liver membranes detected a truncated protein in homozygous mutant animals.|
|Mutations Made By|| |
Dr. Joachim Herz, Univ of Texas Southwest Med Ctr Dallas
This Ldlrtm1Her strain is maintained by mating homozygous siblings. Only homozygous mice may be ordered. Most of these mice have white-bellied agouti coat color.
When using the B6;129S7-Ldlrtm1Her/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #002077 in your Materials and Methods section.