There have been several remutations to Ames waltzer (av) have occurred in stocks at The Jackson Laboratory. All mice homozygous for these remutations show the characteristic head-tossing, circling, and deafness characteristic of Ames waltzer. Ames wa ltzer 2J homozygous mutant mice (av2J/av2J) are more severely afflicted than mice homozygous for the other Jackson (J) remutations. av2J/av2J homozygotes become disoriented and sink in swim tests; Ames waltzer 3J homozygotes (av3J/av3J), like Ames waltzer-J (avJ/avJ), swim with difficulty and circle afloat. In the membranous labyrinth the fluid spaces in the Organ of Corti fail to develop. Later, hair cells and spiral ganglion cells degenerate.
Ames waltzer 3 Jackson (Pcdh15av-3J) arose spontaneously on strain C57BL/6J when it was at F175 at the Jackson Laboratory in the early 1990's. It was maintained on that strain and was cryopreserved in 1993 by mating homozygous males at F175+1N2F1 to C57BL/6J females.
|Allele Name||Ames waltzer 3 Jackson|
|Allele Synonym(s)||av3J; Pcdh15av-3J|
|Gene Symbol and Name||Pcdh15, protocadherin 15|
|Strain of Origin||C57BL/6J|
|General Note||These homozygotes, like Pcdh15av-J homozygotes, can swim, though with difficulty. They tend to circle while afloat (J:28688).|
|Molecular Note||An insertion of a single A nucleotide after position 4521 in the coding region results in a frameshift and a premature stop codon. Any encoded protein is predicted to lack a cytoplasmic domain.|
female homozygotes do breed successfully, but are not part of the optimal breeding scheme.
When using the Ames waltzer 3 Jackson mouse strain in a publication, please cite the originating article(s) and include JAX stock #002072 in your Materials and Methods section.