FVB/N-Tg(HIV-1-luc)326Morr/J

Stock number: 002060
Product name: HIV-1 LTR-luciferase

Description

This reporter transgene, with the HIV-1 LTR fused to luciferase, is valuable in determining what stimuli may induce HIV-1 expression.

Detailed description

This reporter transgene, with the HIV-1 LTR fused to luciferase, is valuable in determining what stimuli may induce HIV-1 expression. Morrey et al, used these mice to show that this promoter sequence responds in the skin to topical application of dimethylsulfoxide, retinoic acid, phorbol ester, ultraviolet light, and hydrogen peroxide, as well as mild wounding but not to arachidonic acid, histamine, ethanol, acetone, or methanol. Exposure of the mice to either UVB radiation or UVA with psoralen, a light-sensitive DNA-binding compound (PUVA therapy), also increased reporter expression.

Development

Sequence from -167 to +80, containing the complete U3 region from the human immunodeficiency virus (HIV-1) long terminal repeat (LTR) from strain HXB2 was linked to the firefly luciferase reporter sequence. This promoter sequence includes NFKB and Sp1 binding sites but excludes the negative regulatory sequence. This was injected into FVB/N fertilized oocytes and lines from several founders were developed. The lines now available from The Jackson Laboratory are stock #002059, FVB/N-Tg(HIV-1)316Morr/J, which has 23 copies of the transgene, and #002060, FVB/N-Tg(HIV-luc)326Morr/J, which has 18 copies. These strains remained coisogenic and in 1992, when they were at approximately generation F7, they were imported from Dr. John D. Morrey, Utah State University in Logan, into The Jackson Laboratory for cryopreservation and distribution. Upon arrival each was sibling intercrossed for expansion for two generations and embryos were generated for cyropreservation from intercrossing homozygotes.

Allele

Symbol: Tg(HIV-1-luc)326Morr
Name: transgene insertion 326, John D Morrey
MGI accession ID: MGI:3789810
Generation method: Transgenic
Attributes: Reporter
Synonyms: HIV-1 LTR-luciferase; Tg(HIV-luc)326Morr
Marker symbol: Tg(HIV-1-luc)326Morr
Marker name: transgene insertion 326, John D Morrey
Marker synonyms: HIV-1 LTR-luciferase; Tg(HIV-luc)326Morr
Chromosome: UN
Strain of origin: FVB/N
Expressed genes: luc,luciferase,firefly
Molecular note: Sequence from -167 to +80 from the human immunodeficiency virus (HIV-1) long terminal repeat (LTR) from strain HXB2 was linked to the firefly luciferase reporter sequence. This promoter sequence includes NFKB and Sp1 binding sites but excludes the negative regulatory sequence. 18 copies inserted into this line.