B6.129-Apoc3^tm1Unc/J

Stock number: 002057
Research areas: Cardiovascular Research

Description

Mice homozygous for this Apoc3 knockout allele exhibit reduced fasted plasma triglyceride, cholesterol and HDL levels.

Detailed description

Mice homozygous for Apoc3^tm1Unc show fasted plasma triglyceride levels that are 70% that present in wildtype siblings. Levels of total plasma cholesterol and HDL in homozygous mice are slightly reduced compared to normal wildtype siblings. Homozygotes also show hypotriglyceridemia when given the "fat meal test", and are able to clear chylomicrons faster than normal wildtype siblings. Heterozygous mice show fasted plasma triglyceride levels that are reduced compared to normal wildtype mice, though not as severely as in homozygous mice.

Development

A targeting vector was designed to replace exon 4 of the apolipoprotein C-III (Apoc3) gene with a neomycin resistance (neo) cassette in reverse orientation to the gene. The construct was electroporated into 129P2/OlaHsd-derived E14TG2a embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric males were bred to C57BL/6J females. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony. These mice were subsequently bred to C57BL/6J mice for at least 9 generations.

Allele

Symbol: Apoc3^tm1Unc
Name: targeted mutation 1, University of North Carolina
MGI accession ID: MGI:1857128
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Apoc3
Marker name: apolipoprotein C-III
Chromosome: 9
Strain of origin: 129P2/OlaHsd
Molecular note: A genomic fragment containing exon 4 was replaced with a neomycin selection cassette. Northern blot analysis indicated that no stable transcript was produced from this allele in homozygous mice. Expression of the Apoa1 and Apoa4 genes that lie in close proximity to Apoc3 are reduced by half in the intestine of homozygotes but not in the liver.