Mice homozygous for this Apoc3 knockout allele exhibit reduced fasted plasma triglyceride, cholesterol and HDL levels.
Dr. Nobuyo Maeda, University of North Carolina at Chapel Hill
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Apoc3 | apolipoprotein C-III |
Mice homozygous for Apoc3tm1Unc show fasted plasma triglyceride levels that are 70% that present in wildtype siblings. Levels of total plasma cholesterol and HDL in homozygous mice are slightly reduced compared to normal wildtype siblings. Homozygotes also show hypotriglyceridemia when given the "fat meal test", and are able to clear chylomicrons faster than normal wildtype siblings. Heterozygous mice show fasted plasma triglyceride levels that are reduced compared to normal wildtype mice, though not as severely as in homozygous mice.
A targeting vector was designed to replace exon 4 of the apolipoprotein C-III (Apoc3) gene with a neomycin resistance (neo) cassette in reverse orientation to the gene. The construct was electroporated into 129P2/OlaHsd-derived E14TG2a embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric males were bred to C57BL/6J females. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony. These mice were subsequently bred to C57BL/6J mice for at least 9 generations.
Allele Name | targeted mutation 1, University of North Carolina |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | apoc3- |
Gene Symbol and Name | Apoc3, apolipoprotein C-III |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 9 |
Molecular Note | A genomic fragment containing exon 4 was replaced with a neomycin selection cassette. Northern blot analysis indicated that no stable transcript was produced from this allele in homozygous mice. Expression of the Apoa1 and Apoa4 genes that lie in close proximity to Apoc3 are reduced by half in the intestine of homozygotes but not in the liver. |
Mutations Made By | Dr. Nobuyo Maeda, University of North Carolina at Chapel Hill |
This strain is maintained by homozygous sibling matings.
When using the B6.129-Apoc3tm1Unc/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #002057 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Apoc3<tm1Unc> |
Frozen Mouse Embryo | B6.129-Apoc3<tm1Unc>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129-Apoc3<tm1Unc>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129-Apoc3<tm1Unc>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6.129-Apoc3<tm1Unc>/J Frozen Embryos | $3373.50 |
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