These NOD-Ead line 5 mice express a transgene containing a construct of the complete H2-Ead gene from BALB/cByJ. This strain is resistant to insulin-dependent diabetes mellitus with overexpression of H2-Ead.
Dr. Edward Leiter, The Jackson Laboratory
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
A requirement for developing insulin-dependent diabetes mellitus (IDDM) in mice is the absence of functional H2-Ea and H2-Ab1 genes. NOD/Lt mice (H2g7 = Kd, Ag7, Enull, Db) fulfill this requirement and develop IDDM. Two homozygous transgenic lines (NOD/ShiLt-Tg(H2-Ead)5Lt/J (Stock No. 002034) commonly referred to as line 5; and NOD/ShiLt-Tg(H2-Ead)12Lt/J (Stock No. 002035), referred to as line 12) were created to further examine the quantitative thresholds of MHC class II expression and the mechanisms involved in preventing IDDM development in NOD mice. These strains harbor a construct containing a complete H2-Ead gene from BALB/cByJ mice. Only a single copy of the Ead transgene integrated into the genome of line 12 where as four copies are present in line 5. Diabetes incidence in line 12 is greatly reduced (25% of females, 0% of males) where H2-Ea expression on antigen presenting cells is comparable to BALB/cByJ controls. Overexpression of H2-Ea in line 5 confers nearly complete resistance to IDDM. Although the presence of the Ead transgene in both lines confers protective influence from IDDM pathologies that affect pancreatic beta cells, autoimmune pathologies effecting other tissues (submandibular salivary, thyroid, kidney) are observed. Histologic examination of thepancreas reveals perivascular/periductal infiltration and peri-insulitus.
A 14 kb XhoI-SacII fragment containing the H2-Ea gene and flanking sequences was isolated from a cosmid of BALB/c origin and utilized in a transgenic construct. The construct was injected into NOD/Lt zygotes. Two founder lines resulted (Stock Nos. 002034 and 002035).
Expressed Gene | H2-I, H2 I region, includes H2-Aa, Ab, Bl, Ea, Eb, Eb2, Ob, Pb, mouse, laboratory |
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Site of Expression |
Allele Name | transgene insertion 5, Edward H Leiter |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | Tg(Ead)Lt |
Gene Symbol and Name | Tg(H2-Ead)5Lt, transgene insertion 5, Edward H Leiter |
Gene Synonym(s) | |
Promoter | H2-I, H2 I region, includes H2-Aa, Ab, Bl, Ea, Eb, Eb2, Ob, Pb, mouse, laboratory |
Expressed Gene | H2-I, H2 I region, includes H2-Aa, Ab, Bl, Ea, Eb, Eb2, Ob, Pb, mouse, laboratory |
Strain of Origin | NOD/ShiLt |
Chromosome | UN |
General Note | Overexpression of H2-Ea in line 5 confers nearly complete resistance to IDDM. Although the presence of the transgene in both lines 5 and 12 confers protective influence from IDDM pathologies that affect pancreatic beta cells, autoimmune pathologies effecting other tissues (submandibular salivary, thyroid, kidney) are observed. Histologic examination of the pancreas reveals perivascular/periductal infiltration and peri-insulitus. |
Molecular Note | The construct contains a complete H2-Ead gene from the BALB/cByJ mouse including flanking sequence. One copy of the transgene integrated into the genome of line 12 whereas four copies are present in line 5. |
This strain is maintained by homozygous sibling matings. There have been no reproductive performance problems reported. Expected coat color from breeding:albino
When using the NOD-Eαd Line 5 mouse strain in a publication, please cite the originating article(s) and include JAX stock #002034 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Homozygous for Tg(H2-Ea<d>)5Lt, 1 pair minimum |
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