NOD/ShiLt-Tg(Ins2-TAg)1Lt/J

Stock number: 002033
Product name: NOD RipTag
Strain synonyms: NOD/Lt-Tg(Ins2-TAg)1Lt
Research areas: Cancer Research, Diabetes and Obesity Research

Description

Homozygous NOD RipTag mice (NOD/Lt-Tg(RipTag)1Lt) develop pancreatic beta cell adenomas.

Detailed description

Homozygous NOD RipTag mice (NOD/Lt-Tg(RipTag)1Lt) develop pancreatic beta cell adenomas. They are able to produce 2-3 litters before the insulin secreted from the adenoma makes them too hypoglycemic. If needed, transgenic mice can be supplemented with 5% sucrose in the drinking water to limit the development of hypoglycemia.

Development

The transgene contains rat insulin 2 promoter (Rip) and the wild-type allele of the SV40 large T antigen (TAg) gene. The recombinant DNA was microinjected into male pronuclei of NOD/Lt zygotes. Injected two-cell embryos were transferred into the uteri of pseudopregnant B6 x SJL F1 foster mothers. Founder females were sib mated to establish the line.

Allele

Symbol: Tg(Ins2-TAg)1Lt
Name: transgene insertion 1, Edward H Leiter
MGI accession ID: MGI:3052662
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(Ins2-TAg)1Lt
Marker name: transgene insertion 1, Edward H Leiter
Chromosome: UN
Strain of origin: NOD/ShiLt
Expressed genes: TAg,SV40 large T-antigen,SV40
Molecular note: The transgene contains rat insulin 2 promoter (Rip) and the wild-type allele of the SV40 large T antigen (TAg) gene.
General note: Homozygous transgenic mice on an NOD/Lt genetic background develop pancreatic beta cell adenomas. They are able to produce 2-3 litters before the insulin secreted from the adenoma makes them too hypoglycemic.