C57BL/6-Tg(Igh-3/Igh-6VD93)1241Ust/J

Stock number: 002030
Product name: γ2b-μmem
Research areas: Immunology, Inflammation and Autoimmunity Research

Description

These γ2b-μmem mice express a transgene containing the variable and constant regions from mouse gamma2b (Igh-3) clone (VD93) fused to membrane exons from the mu (Igh-6) gene.

Detailed description

Transgenic gamma 2b is present on the surface of B cells. Young transgenic mice have a dramatic decrease in B cell numbers, however, older mice have almost normal B cell numbers. All gamma 2b-expressing B cells in the spleen also express mu. Presence of the gamma 2b transgene strongly enhances the feedback inhibition of endogenous heavy chain gene rearrangement. Light chain genes are expressed normally, and the early expression of transgenic light chains does not improve B cell maturation.

Development

This transgenic strain was sent to The Jackson Laboratory from the laboratory of Dr. Ursula Storb of the University of Chicago by NIAID in 1992 for cryopreservation at an unspecified generation. Embryos for cryopreservation were produced by mating transgenic males to C57BL/6J females.

Allele

Symbol: Tg(Igh-3/Igh-6VD93)1241Ust
Name: transgene insertion 1241 Ursula Storb
MGI accession ID: MGI:3793439
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(Igh-3/Igh-6VD93)1241Ust
Marker name: transgene insertion 1241 Ursula Storb
Chromosome: UN
Strain of origin: C57BL/6
Expressed genes: Ighm,immunoglobulin heavy constant mu,mouse, laboratory; Ighg2b,immunoglobulin heavy constant gamma 2B,mouse, laboratory
Molecular note: The transgene construct consists of the variable and constant regions from mouse gamma2b (Igh-3) clone (VD93) fused to membrane exons from the mu (Igh-6) gene (2.6kb fragment from pVH176mu).