C57BL/6-Tg(Igl-2MOPC315)1275Ust/J

Stock number: 002029
Product name: λ2
Research areas: Immunology, Inflammation and Autoimmunity Research

Description

These λ2 mice express a transgene containing a functionally arranged lambda 2 (Igl-2) gene from clone MOPC315 under the control of the heavy chain enhancer (mu J-C intron).

Detailed description

This lambda 2 transgene with the H enhancer is expressed in lymphoid cells only. The majority of the B cells of newborn transgenic mice produce lambda, whereas kappa + cells are reduced., Serum levels of kappa and kappa mRNA are diminished. By 2 weeks after birth the proportion of kappa-expressing cells is dramatically increased. Adults have reduced proportions of B cells that produce lambda only, but the levels of lambda are still higher than in normal littermates. Also, kappa + cells are still lower than in normal mice. Analysis of hybridomas reveals that reduction of kappa gene rearrangement is the basis for the decreased frequency of kappa + cells. Furthermore, many cells also contain an un-rearranged H chain allele.

Development

This transgenic strain was sent to The Jackson Laboratory from the laboratory of Dr. Ursula Storb of the University of Chicago by NIAID in 1992 for cryopreservation at an unspecified generation. Embryos for cryopreservation were produced by mating transgenic males to C57BL/6J females.

Allele

Symbol: Tg(Igl-2MOPC315)1275Ust
Name: transgene insertion 1275, Ursula Storb
MGI accession ID: MGI:3793466
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(Igl-2MOPC315)1275Ust
Marker name: transgene insertion 1275, Ursula Storb
Chromosome: UN
Strain of origin: C57BL/6
Expressed genes: Igl-2,immunoglobulin lambda chain, cluster 2,mouse, laboratory
Molecular note: The transgene construct contains a functionally arranged lambda 2 (Igl-2) gene from clone MOPC315 under the control of the heavy chain enhancer (mu J-C intron).