C3A.BLiA-Pde6b⁺/J

Stock number: 001912
Strain synonyms: C3H/f<+/+>
Research areas: Mouse/Human Gene Homologs, Sensorineural Research

Description

In this congenic strain, the wild type Pde6b allele from C57BL/LiA was introgressed onto the C3Hf/HeA background. Retinal degeneration 1 (Pde6b^rd1) is a spontaneous mutation that is present in multiple inbred strains, including the C3H substrains. Strains that carry rd1 lack rod cells. This strain provides a sighted C3H mouse for use in research studies that require normal vision.

Detailed description

In this congenic strain, the wild type Pde6b allele from C57BL/LiA was introgressed onto the C3Hf/HeA background. Retinal degeneration 1 (Pde6b^rd1) is a spontaneous mutation that is present in multiple inbred strains, including the C3H substrains. Strains that carry rd1 lack rod cells. This strain provides a sighted C3H mouse for use in research studies that require normal vision. This strain also does not have the nob5 allele of Gpr179 (Chang, 2015) or the Clcc1^m1J allele found in C3H/HeSn subline mice.

Development

This strain was developed in the laboratory of Willem J. de Grip at Erasmus Universiteit, Rotterdam, the Netherlands. An initial cross of C3Hf/HeA with C57BL/LiA was followed by 8 generations of backcrossing to C3Hf/HeA to introgress the wild type Pde6b allele of C57BL/LiA onto the C3Hf/HeA background. The heterozygous progeny of the last backcross were intercrossed to produce homozygous wild type (Pde6b⁺/Pdeb⁺) mice, after which the strain was maintained by sib inbreeding. The strain had exceeded 30 generations of sibling mating when it was sent to The Jackson Laboratory in 1991. (Schalken et al., 1990 and TJL documentation)

Additional backgrounds available: using this strain as the donor, the wild type allele of Pde6b was backcrossed for 10 generations to C3H/HeSnJ. This was done for the purpose of generating a hardier control/background strain for use in studies of additional retinal degeneration mutations. The product of this backcross, C3Sn.BLiA-Pde6b⁺/Dn (Stock No. 003648), is now maintained by sib mating.

Allele

Symbol: Pde6b⁺
Name: wild type
MGI accession ID: MGI:2430003
Generation method: Not Applicable
Marker symbol: Pde6b
Marker name: phosphodiesterase 6B, cGMP, rod receptor, beta polypeptide
Marker synonyms: CSNB3; CSNBAD2; GMP-PDEbeta; nmf137; PDEB; r; rd; rd1; rd10; RP40
Chromosome: 5
Strain of origin: Not Applicable
Expressed genes: Pde6b,phosphodiesterase 6B, cGMP, rod receptor, beta polypeptide,mouse, laboratory
Site of expression: Retina.