These I-FABP-hGH mice contain a transgene expressing human growth hormone controlled by a partial rat intestinal fatty acid-binding protein promoter with expression restricted to the intestine.Read More +
Obligate hemizygous F1 offspring from founder 54 carried approximately 60 copies of the transgene in tandem head-to-tail arrangement in a single insertion site. Expression of the reporter gene, human growth hormone, is appropriately restricted to the intestine where the duodenum and proximal jejunum show expression levels normal for intestinal fatty acid binding protein, but the distal small intestine shows reduced expression. Resultant serum human growth hormone levels were 6-38 ng/ml which is approximately 1000 times lower than in mice carrying a transgene with an extended I-FABP promoter which includes nucleotides -1178 to +28. Use of the extended (-1178 to +28) promoter also results in a more normal expression in the distal small intestine. (Sweetser et al., 1988.)
Nucleotides -277 to +28 of the promoter of rat intestinal fatty acid binding protein (I-FABP), referred to as the short promoter sequence, were fused to nucleotide +3 of the human growth hormone (GH) gene and microinjected into the pronuclei of zygotes generated by breeding either C57BL/6J females or (B6SJL)F1 females with LT/Sv or (B6SJL)F1 males. (Sweetser et al., 1988)
Currently there are no related genes or alleles for this strain.