| Allele Name | transgene insertion 54, Diane Mathis |
| Allele Type | Transgenic (Inserted expressed sequence) |
| Allele Synonym(s) | Tg(dY301)3Strasbourg; Tg(dY301,54)3Strasbourg; WEdeltaY301; WEdeltaY301-54 |
| Gene Symbol and Name | Tg(H2-Ea*deltaY)54Dim, transgene insertion 54, Diane Mathis |
| Gene Synonym(s) | |
| Promoter | H2-I, H2 I region, includes H2-Aa, Ab, Bl, Ea, Eb, Eb2, Ob, Pb, mouse, laboratory |
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| Expressed Gene | H2-I, H2 I region, includes H2-Aa, Ab, Bl, Ea, Eb, Eb2, Ob, Pb, mouse, laboratory |
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| Strain of Origin | (C57BL/6 x SJL)F2 |
| Chromosome | UN |
| Molecular Note | A 9 kb fragment of the H2-Ea(k) from the A/J mouse (clone lambdaA/J p34.13) was modified at 3 positions creating the WE32 construct (modified H2-Ea). This was used as the basis for a mutant H2-Ea gene having a deletion of the Y box (from position -61 to position -48). The resulting 8 kb transgenic construct WEdeltaY301 was injected into (C57BL/6 x SJL)F2 embryo pronuclei. Multiple lines - 22, 45, 54, 58, and 59, were derived, and 54 was donated to The Jackson Laboratory. S1 nuclease mapping shows that little H2-Ea mRNA is expressed in the spleen. Initiation of transcripts is aberrant. |
