These transgenic mice carry H2-I Eα genes deleted for the Y box.
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
These transgenic mice carry H2-I Eα genes deleted for the Y box.
Expressed Gene | H2-I, H2 I region, includes H2-Aa, Ab, Bl, Ea, Eb, Eb2, Ob, Pb, mouse, laboratory |
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Site of Expression |
Allele Name | transgene insertion 54, Diane Mathis |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | Tg(dY301)3Strasbourg; Tg(dY301,54)3Strasbourg; WEdeltaY301; WEdeltaY301-54 |
Gene Symbol and Name | Tg(H2-Ea*deltaY)54Dim, transgene insertion 54, Diane Mathis |
Gene Synonym(s) | |
Promoter | H2-I, H2 I region, includes H2-Aa, Ab, Bl, Ea, Eb, Eb2, Ob, Pb, mouse, laboratory |
Expressed Gene | H2-I, H2 I region, includes H2-Aa, Ab, Bl, Ea, Eb, Eb2, Ob, Pb, mouse, laboratory |
Strain of Origin | (C57BL/6 x SJL)F2 |
Chromosome | UN |
Molecular Note | A 9 kb fragment of the H2-Ea(k) from the A/J mouse (clone lambdaA/J p34.13) was modified at 3 positions creating the WE32 construct (modified H2-Ea). This was used as the basis for a mutant H2-Ea gene having a deletion of the Y box (from position -61 to position -48). The resulting 8 kb transgenic construct WEdeltaY301 was injected into (C57BL/6 x SJL)F2 embryo pronuclei. Multiple lines - 22, 45, 54, 58, and 59, were derived, and 54 was donated to The Jackson Laboratory. S1 nuclease mapping shows that little H2-Ea mRNA is expressed in the spleen. Initiation of transcripts is aberrant. |
When using the WEΔY301-54 mouse strain in a publication, please cite the originating article(s) and include JAX stock #001812 in your Materials and Methods section.
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Carrier or non-carrier for Tg(H2-Ea*deltaY)54Dim |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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