STOCK Tg(H2-Ea*deltaY)54Dim/J

Stock number: 001812
Product name: WEΔY301-54
Research areas: Research Tools

Detailed description

These transgenic mice carry H2-I Eα genes deleted for the Y box.

Allele

Symbol: Tg(H2-Ea*deltaY)54Dim
Name: transgene insertion 54, Diane Mathis
MGI accession ID: MGI:3793482
Generation method: Transgenic
Attributes: Inserted expressed sequence
Synonyms: Tg(dY301)3Strasbourg; Tg(dY301,54)3Strasbourg; WEdeltaY301; WEdeltaY301-54
Marker symbol: Tg(H2-Ea*deltaY)54Dim
Marker name: transgene insertion 54, Diane Mathis
Marker synonyms: Tg(dY301)3Strasbourg; Tg(dY301,54)3Strasbourg; WEdeltaY301; WEdeltaY301-54
Chromosome: UN
Strain of origin: (C57BL/6 x SJL)F2
Expressed genes: H2-I,H2 I region, includes H2-Aa, Ab, Bl, Ea, Eb, Eb2, Ob, Pb,mouse, laboratory
Molecular note: A 9 kb fragment of the H2-Ea(k) from the A/J mouse (clone lambdaA/J p34.13) was modified at 3 positions creating the WE32 construct (modified H2-Ea). This was used as the basis for a mutant H2-Ea gene having a deletion of the Y box (from position -61 to position -48). The resulting 8 kb transgenic construct WEdeltaY301 was injected into (C57BL/6 x SJL)F2 embryo pronuclei. Multiple lines - 22, 45, 54, 58, and 59, were derived, and 54 was donated to The Jackson Laboratory. S1 nuclease mapping shows that little H2-Ea mRNA is expressed in the spleen. Initiation of transcripts is aberrant.