STOCK Tg(H2-Ea)16Dim/J

Stock number: 001658
Product name: Eα16
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Detailed description

These Eα16 mice express a transgene containing all the mouse (A/J) H2-Ea kappa exons. Twelve copies of the transgene are carried in the genome.

Allele

Symbol: Tg(H2-Ea)16Dim
Name: transgene insertion 16, Diane Mathis
MGI accession ID: MGI:3793709
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(H2-Ea)16Dim
Marker name: transgene insertion 16, Diane Mathis
Chromosome: UN
Strain of origin: Not Applicable
Expressed genes: H2-I,H2 I region, includes H2-Aa, Ab, Bl, Ea, Eb, Eb2, Ob, Pb,mouse, laboratory
Molecular note: An 8.2 kb construct containing all the mouse (A/J) H2-Ea kappa exons (from clone lambda Charon 4) plus 2 kb of DNA 5' to the cap site and 1.4 kb 3' to the poly(A) addition site was microinjected into (C57BL/6 x SJL) embryos. Two lines 23 and 16 were generated with line 16 carrying 12 copies of the transgene in its genome and line 23 having 4 copies. Northern blots and S1 nuclease analysis showed transcripts in the spleen. Line 16 produced more transcripts than line 4, and both lines showed correct initiation sites of transcription.
General note: Transgenic lines were derived from two transgenic founder mice and designated line 16 and line 23. Line 16 exhibits slightly higher antibody titers and mRNA levels in response to synthetic antigen.