| Allele Name | transgene insertion 16, Diane Mathis |
| Allele Type | Transgenic (Inserted expressed sequence) |
| Allele Synonym(s) | Ea16; Tg(Ea16)1Strasbourg |
| Gene Symbol and Name | Tg(H2-Ea)16Dim, transgene insertion 16, Diane Mathis |
| Gene Synonym(s) | |
| Promoter | H2-I, H2 I region, includes H2-Aa, Ab, Bl, Ea, Eb, Eb2, Ob, Pb, mouse, laboratory |
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| Expressed Gene | H2-I, H2 I region, includes H2-Aa, Ab, Bl, Ea, Eb, Eb2, Ob, Pb, mouse, laboratory |
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| Strain of Origin | Not Applicable |
| Chromosome | UN |
| General Note | Transgenic lines were derived from two transgenic founder mice and designated line 16 and line 23. Line 16 exhibits slightly higher antibody titers and mRNA levels in response to synthetic antigen. |
| Molecular Note | An 8.2 kb construct containing all the mouse (A/J) H2-Ea kappa exons (from clone lambda Charon 4) plus 2 kb of DNA 5' to the cap site and 1.4 kb 3' to the poly(A) addition site was microinjected into (C57BL/6 x SJL) embryos. Two lines 23 and 16 were generated with line 16 carrying 12 copies of the transgene in its genome and line 23 having 4 copies. Northern blots and S1 nuclease analysis showed transcripts in the spleen. Line 16 produced more transcripts than line 4, and both lines showed correct initiation sites of transcription. |
