These Eα16 mice express a transgene containing all the mouse (A/J) H2-Ea kappa exons. Twelve copies of the transgene are carried in the genome.
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
These Eα16 mice express a transgene containing all the mouse (A/J) H2-Ea kappa exons. Twelve copies of the transgene are carried in the genome.
Expressed Gene | H2-I, H2 I region, includes H2-Aa, Ab, Bl, Ea, Eb, Eb2, Ob, Pb, mouse, laboratory |
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Site of Expression |
Allele Name | transgene insertion 16, Diane Mathis |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | Ea16; Tg(Ea16)1Strasbourg |
Gene Symbol and Name | Tg(H2-Ea)16Dim, transgene insertion 16, Diane Mathis |
Gene Synonym(s) | |
Promoter | H2-I, H2 I region, includes H2-Aa, Ab, Bl, Ea, Eb, Eb2, Ob, Pb, mouse, laboratory |
Expressed Gene | H2-I, H2 I region, includes H2-Aa, Ab, Bl, Ea, Eb, Eb2, Ob, Pb, mouse, laboratory |
Strain of Origin | Not Applicable |
Chromosome | UN |
General Note | Transgenic lines were derived from two transgenic founder mice and designated line 16 and line 23. Line 16 exhibits slightly higher antibody titers and mRNA levels in response to synthetic antigen. |
Molecular Note | An 8.2 kb construct containing all the mouse (A/J) H2-Ea kappa exons (from clone lambda Charon 4) plus 2 kb of DNA 5' to the cap site and 1.4 kb 3' to the poly(A) addition site was microinjected into (C57BL/6 x SJL) embryos. Two lines 23 and 16 were generated with line 16 carrying 12 copies of the transgene in its genome and line 23 having 4 copies. Northern blots and S1 nuclease analysis showed transcripts in the spleen. Line 16 produced more transcripts than line 4, and both lines showed correct initiation sites of transcription. |
When using the Eα16 mouse strain in a publication, please cite the originating article(s) and include JAX stock #001658 in your Materials and Methods section.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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