STOCK l1Rk4/J

Stock number: 001641
Product name: Lethal 4

Detailed description

This stock carries a triethylene melamine induced lethal mutation made from mice carrying the In(1)1Rk inversion (Stock No. 000716). The lethal mutation in I1Rk4 acts at birth or pre-implantation and is thought to be located within the inverted segment (Roderick, 1983).

Development

A male homozygous for the inversion In(1)1Rk was treated with triethylene melamine (TEM) and subsequently bred to a female heterozygous for splotch and homozygous for leaden (Pax3^Sp Mlph^ln/Pax3⁺ Mlph^ln). Splotch and leaden are within the inverted segment in In(1)1Rk and thus do not recombine when in repulsion with this inversion. The offspring that expressed the splotch mutation were thus heterozygous for splotch and leaden in repulsion with In(1)1Rk and any recessive lethal mutation induced by the TEM. When sibling intercrossed these splotch expressing repulsion heterozygotes generate embryos homozygous for splotch, which are in utero lethal, homozygous for any induced recessive mutation, and the same genotype as the parental animals, Pax3^Sp Mlph^ln/In(1)1Rk l1Rk4. This is how the l1Rk4 mutation was induced, isolated, and maintained prior to cryopreservation. Embryos were generated for cryopreservation from Pax3^Sp Mlph^ln/In(1)1Rk l1Rk4 males bred with either Pax3^Sp Mlph^ln/Pax3⁺ Mlph^ln or Pax3⁺ Mlph^ln/Pax3⁺ Mlph^ln females. The cryorecovered pups that do not have the leaden dilution of the coat color but do have the spots of the splotch mutation can be expected to be carriers of the recessive lethal l1Rk4 mutation. No crossover events were observed in 285 mice. The recovered line can be maintained by intercrossing these carriers.

Allele

Symbol: l1Rk4
Name: lethal, Chr 1, Roderick 4
MGI accession ID: MGI:3027873
Generation method: Chemically induced (other)
Marker symbol: l1Rk4
Marker name: lethal, Chr 1, Roderick 4
Marker synonyms: l(1)-4Rk
Chromosome: 1
Strain of origin: C57BL/6J x DBA/2J
Molecular note: This mutation was induced by exposure of males with an inversion on chromosome 1 to 0.3mg/kg of triethylene melamine.